Pathogenic infection characteristics and risk factors for bovine respiratory disease complex based on the detection of lung pathogens in dead cattle in Northeast China
文献类型: 外文期刊
第一作者: Zhou, Y.
作者: Zhou, Y.;Dai, G.;Li, X.;Xiang, Y.;Jiang, S.;Zhang, Z.;Zhu, Z.;Fan, C.;Zhou, Y.;Zhou, Y.;Shao, Z.;Ren, Y.;Zhang, G.
作者机构:
关键词: bovine respiratory disease complex; necropsy; pathogen detection; epidemiological investigation
期刊名称:JOURNAL OF DAIRY SCIENCE ( 影响因子:3.5; 五年影响因子:4.2 )
ISSN: 0022-0302
年卷期: 2023 年 106 卷 1 期
页码:
收录情况: SCI
摘要: Bovine respiratory disease complex (BRDC) involves multiple pathogens, shows diverse lung lesions, and is a major concern in calves. Pathogens from 160 lung samples of dead cattle from 81 cattle farms in Northeast China from 2016 to 2021 were collected to characterize the molecular epidemiology and risk factors of BRDC and to assess the major pathogens involved in bovine suppurative or caseous necrotizing pneumonia. The BRDC was diagnosed by autopsy, pathogen isolation, PCR, or reverse transcription-PCR detection, and gene sequencing. More than 18 species of pathogens, including 491 strains of respiratory pathogens, were detected. The positivity rate of bacteria in the 160 lung samples was 31.77%, including Trueperella pyogenes (9.37%), Pasteurella multocida (8.35%), Histophilus somni (4.48%), Mannheimia haemolytica (2.44%), and other bacteria (7.13%). The positivity rate of Mycoplasma spp. was 38.9%, including M. bovis (7.74%), M. dispar (11.61%), M. bovirhinis (7.94%), M. alkalescens (6.11%), M. arginini (0.81%), and undetermined species (4.68%). Six species of viruses were detected with a positivity rate of 29.33%, including bovine herpesvirus-1 (BoHV1; 13.25%), bovine respiratory syncytial virus (BRSV; 5.50%), bovine viral diarrhea virus (BVDV; 4.89%), bovine parainfluenza virus type-3 (BPIV-3; 4.28%), bovine parainfluenza virus type-5 (1.22%), and bovine coronavirus (2.24%). Mixed infections among bacteria (73.75%), viruses (50%), and M. bovis (23.75%) were the major features of BRDC in these cattle herds. The risk analysis for multi-pathogen co-infection indicated that BoHV-1 and H. somni; BVDV and M. bovis, P. multocida, T. pyogenes, or Mann. haemolytica; BPIV-3 and M. bovis; BRSV and M. bovis, P. multocida, or T. pyogenes; P. multocida and T. pyogenes; and M. bovis and T. pyogenes or H. somni showed co-infection trends. A survey on molecular epidemiology indicated that the occurrence rate of currently prevalent pathogens in BRDC was 46.15% (6/13) for BoHV-1.2b and 53.85% (7/13) for BoHV-1.2c, 53.3% (8/15) for BVDV-1b and 46.7% (7/15) for BVDV-1d, 29.41% (5/17) for BPIV-3a and 70.59% (12/17) for BPIV-3c, 100% (2/2) for BRSV gene subgroup IX, 91.67% (33/36) for P. multocida serotype A, and 8.33% (3/36) for P. multocida serotype D. Our research discovered new subgenotypes for BoHV-1.2c, BRSV gene subgroup IX, and P. multocida serotype D in China's cattle herds. In the BRDC cases, bovine suppurative or caseous necrotizing pneumonia was highly related to BVDV [odds ratio (OR) = 4.18; 95% confidence interval (95% CI): 1.6-10.7], M. bovis (OR = 2.35; 95% CI: 1.1-4.9), H. somni (OR = 8.2; 95% CI: 2.6-25.5), and T. pyogenes (OR = 13.92; 95% CI: 5.8-33.3). The risk factor analysis found that dairy calves <3 mo and beef calves >3 mo (OR = 5.39; 95% CI: 2.7-10.7) were more susceptible to BRDC. Beef cattle were more susceptible to bovine suppurative or caseous necrotizing pneumonia than dairy cattle (OR = 2.32; 95% CI: 1.2-4.4). These epidemiological data and the new pathogen subgenotypes will be helpful in formulating strategies of control and prevention, developing new vaccines, improving clinical differential diagnosis by necropsy, predicting the most likely pathogen, and justifying antimicrobial use.
分类号:
- 相关文献
作者其他论文 更多>>
-
The jigsaw puzzle of pedigree: whole-genome resequencing reveals genetic diversity and ancestral lineage in Sunong black pigs
作者:Ye, X. W.;Gu, J. M.;Cao, C. Y.;Zhang, Z. Y.;Cheng, H.;Zhang, Z.;Wang, Q. S.;Pan, Y. C.;Wang, Z.;Chen, Z.;Fang, X. M.
关键词:Genomic analysis; Hybrid pigs; Local ancestry inference; Pig breeding; Population structure
-
Comprehensive proteomic analysis of the main liver and attached liver of Glyptosternum maculatum on the basis of data-independent mass spectrometry acquisition
作者:Zhang, G.;Zhang, G.;Mou, Z.;Liu, H.;Wang, H.
关键词:attached liver; data independent; acquisition; Glyptosternum maculatum; main liver; proteomics
-
Endophytic fungi in green manure crops; friends or foe?
作者:Abeywickram, P. D.;Qian, N.;Zhang, W.;Guo, K.;Zhao, W.;Abeywickram, P. D.;Qian, N.;Zhang, W.;Guo, K.;Zhao, W.;Abeywickram, P. D.;Li, Y.;Zhang, W.;Yan, J.;Li, X.;Abeywickram, P. D.;Jayawardena, R. S.;Abeywickram, P. D.;Jayawardena, R. S.;Hyde, K. D.;Zhang, L.;Zhang, G.;Guo, Z.;Peng, Y.
关键词:7 new taxa; Checklist; Culture dependent; Cover crops; High-throughput; sequencing; Taxonomy
-
Improve the accuracy of chicken leg disease assessment by combination of gait score and X-ray scan
作者:Zheng, J.;Zhang, G.;Ding, J.;He, Z.;Li, Q.;Liu, R.;Wen, J.;Zhao, G.;Liu, D.
关键词:white feather broiler; gait score; X-ray; leg health; growth trait
-
Protective effect of sesamol on cognitive impairment in APP/PS1 mice
作者:Xu, Y. L.;Zhang, Y. D.;Wang, Z. P.;Wang, T.;Rong, S.;Chen, W. W.;Fan, C.;Wang, T.;Xu, J. Q.
关键词:sesamol; Alzheimer's disease; cognition; SIRT1; BDNF
-
The Effect of a-Fe2O3 Nanoparticles on the Physiological Characteristics and Active Ingredients of Muskmelon on the Cadmium-Polluted Soil
作者:Zou, Z.;Zhou, Y.;Hao, Y.;Li, J.;Qi, M.;Qiu, L.;Cheng, Y.;Wang, Y.;Dai, Z.;Wang, Y.;Dai, Z.
关键词:Cucumis melo L.; soil cadmium pollution; muskmelon; & alpha;-Fe2O3 nanoparticles; growth parameters; antioxidant system; active ingredients
-
Genome- Wide Association Study of Abdominal Fat in Wenshang Barred Chicken Based on the Slaf- Seq Technology
作者:Liu, W.;Liu, J.;Zhou, Y.;Cao, D.;Lei, Q.;Han, H.;Wang, J.;Li, D.;Gao, J.;Li, H.;Li, F.;Liu, W.;Liu, J.;Zhou, Y.;Cao, D.;Lei, Q.;Han, H.;Wang, J.;Li, D.;Gao, J.;Li, F.
关键词:Abdominal fat traits; genome-wide association study; SLAF-seq; Wenshang Barred chicken