CONSTANS-LIKE 1a positively regulates salt and drought tolerance in soybean
文献类型: 外文期刊
第一作者: Xu, Chongjing
作者: Xu, Chongjing;Shan, Jinming;Liu, Tianmeng;Ji, Yujia;Zhang, Yuntong;Wang, Mengyuan;Xia, Ning;Zhao, Lin;Wang, Qi
作者机构:
期刊名称:PLANT PHYSIOLOGY ( 影响因子:7.4; 五年影响因子:8.7 )
ISSN: 0032-0889
年卷期: 2023 年 191 卷 4 期
页码:
收录情况: SCI
摘要: The Glycine max CONSTANS-like 1a transcription factor positively regulates tolerance to salt and drought stresses by promoting the accumulation of Delta(1)-pyrroline-5-carboxylate synthetase. Salt and drought stresses are major factors limiting soybean (Glycine max [L.] Merr.) growth and development; thus, improving soybean stress tolerance is critical. In this study, both salt stress and drought stress induced mRNA levels of CONSTANS-like 1a (GmCOL1a) and stabilized the GmCOL1a protein. Transgenic 35S:GmCOL1a soybean plants exhibited enhanced salt and drought tolerance, with higher relative water content in leaves, greater proline content, lower malondialdehyde (MDA) content, and less reactive oxygen species (ROS) production compared with wild-type plants; the GmCOL1a knockout co-9 mutant showed opposite phenotypes. In addition, GmCOL1a promoted the expression of genes related to salt tolerance, effectively reducing the Na+/K+ ratio in soybean plants, especially in stems and leaves of 35S:GmCOL1a soybean. Chromatin immunoprecipitation sequencing (ChIP-seq) analysis identified two potential direct targets of GmCOL1a, late embryogenesis abundant (GmLEA) and Delta(1)-pyrroline-5-carboxylate synthetase (GmP5CS) genes, which were verified by chromatin immunoprecipitation quantitative polymerase chain reaction (ChIP-qPCR), electrophoretic mobility shift assay (EMSA), and transient transcriptional activation assays. GmCOL1a bound directly to the Myc(bHLH)-binding and Che-binding motifs of GmLEA and GmP5CS promoters to stimulate mRNA expression. Analysis of transgenic hairy-root GmP5CS:GmP5CS soybean plants in wild type, co-9, and 35S:GmCOL1a backgrounds further revealed that GmCOL1a enhances salt and drought tolerance by promoting GmP5CS protein accumulation in transgenic soybean hairy roots. Therefore, we demonstrate that GmCOL1a plays an important role in tolerance to abiotic stress in soybean.
分类号:
- 相关文献
作者其他论文 更多>>
-
Genetic mechanisms of petal morphogenesis in Eustoma grandiflorum
作者:Liang, Yuwei;Fang, Huiyi;Wang, Qi;Sun, Fuhui;Bi, Bo;Zhang, Liangsheng;Zhao, Peng;Liu, Bin;Sun, Dan;Ruan, Jiwei;Jin, Chunlian;Li, Fan;Zhao, Peng;Liu, Bin;Zhang, Liangsheng;Bi, Bo
关键词:Eustoma grandiflorum; petal morphogenesis; transcriptional analysis; WGCNA; qRT-PCR; YAB3; LFY
-
Mixed cropping with duck co-culture: an agroecological system to improve grain quality and farm profitability
作者:Zhang, Jiaen;Li, Meijuan;Li, Ronghua;Zhang, Jiaen;Liu, Shiwei;Hei, Zewen;Wang, Qi;Li, Meijuan;Li, Ronghua;Zhang, Jiaen;Li, Ronghua;Zhang, Jiaen;Li, Ronghua;Zhang, Jiaen;Zhang, Jiaen
关键词:mixed cropping; rice-duck co-culture; genetic diversity; species diversity; grain quality; economic benefit
-
Identification of co-expressed networks and key genes associated with organic acid in peach fruit
作者:Wang, Qi;Cao, Ke;Li, Yong;Wu, Jinlong;Fan, Jiaqi;Ding, Tiyu;Khan, Irshad Ahmad;Wang, Lirong
关键词:Organic acid; WGCNA; Transcription factor; Hydrogen ion transportation; Peach
-
MFS Transporter Bdorwp Does Not Affect Antennal Electrophysiology but Regulates Reproductive Behaviors in Bactrocera dorsalis
作者:Zhang, Yan;Wuyun, Qiqige;Wang, Qi;Luo, Zhicai;Zhang, Jie;Yan, Shanchun;Yuan, Jinxi;Liu, Wei;Wang, Guirong
关键词:Bactrocera dorsalis; selectablemarker gene; Bdorwp; CRISPR/Cas9; moleculartargets
-
Enhancing Soil Nitrogen Retention Capacity by Biochar Incorporation in the Acidic Soil of Pomelo Orchards: The Crucial Role of pH
作者:Qian, Xiaojie;Chen, Hongmei;Yi, Zhigang;Li, Qinghua;Zhao, Lin;Wang, Fei;Zhang, Yushu;Zhang, Jinbo;Zhang, Jinbo;Mueller, Christoph;Mueller, Christoph;Mueller, Christoph;Mueller, Christoph
关键词:nitrogen retention capacity; soil pH; biochar incorporation; TM; TI; O-NH4
-
Organ-enriched gene expression during floral morphogenesis in wild barley
作者:Chen, Gang;Mishina, Kohei;Zhu, Hongjing;Tagiri, Akemi;Oono, Youko;Komatsuda, Takao;Chen, Gang;Zhu, Hongjing;Kikuchi, Shinji;Sassa, Hidenori;Oono, Youko;Komatsuda, Takao;Wang, Qi;Komatsuda, Takao
关键词:transcription profiling; floral organs; MADS-box; ABCDE model; co-expression regulatory network
-
Effects of Bee Pollen Derived from Acer mono Maxim. or Phellodendron amurense Rupr. on the Lipid Composition of Royal Jelly Secreted by Honeybees
作者:Zhou, Enning;Wang, Qi;Niu, Qingsheng;Zhou, Enning;Li, Xiangxin;Li, Qiangqiang;Wu, Liming;Zhu, Dan
关键词:royal jelly; bee pollen; lipidomics; UPLC-Q-Exactive Orbitrap mass spectrometry