Establishment of a DNA Barcoding Database for Legume and Grass Species Identification
文献类型: 外文期刊
第一作者: Li, Yongqing
作者: Li, Yongqing;La, Zunongjiang A. Bu;Cao, Lijun;Zhao, Shengguo
作者机构:
关键词: DNA barcoding; Forages; MatK; RbcL; Universal sequences
期刊名称:LEGUME RESEARCH ( 影响因子:0.669; 五年影响因子:0.762 )
ISSN: 0250-5371
年卷期: 2022 年 45 卷 6 期
页码:
收录情况: SCI
摘要: Background: DNA barcoding, an emerging approach, is being widely used to accurately and quickly identify species using conserved DNA sequences. Methods: Herein we designed seven universal primers for matK (matK1, matK2, matK3 and matK4), rbcL, psbA-trnH and ITS based on their nucleotide sequences in GenBank to amplify 40 species of leguminosae and grass forages. Sequence alignment was performed using MEGA 5.0 and haplotype and mutation sites were analyzed with DnaSP 5.10. PCR amplification efficiency on using the primers designed for psbA-trnH and ITS was relatively low, making these sequences unsuitable for DNA barcoding. Further, we optimized target fragment amplification conditions for all 40 species analyzed in this study. On purifying, sequencing and analysing amplification products, we selected 5' - and 3' -end conserved fragments in four marked fragments. Result: Sequences of each maker loci showed that there were 12, 17 and 6 haplotypes of matK1, matK2 and matK3, respectively and 13 of rbcL. Based on these haplotypes of matK1, matK2, matK3 and rbcL, we established a DNA barcoding database for 20 forage species.
分类号:
- 相关文献
作者其他论文 更多>>
-
Dissecting a mutualistic interaction involving an insect-endosymbiont association
作者:Fang, Jie;Wang, Yongqiang;Hu, Jiayao;Li, Lizhen;Yin, Ziwei;Yang, Fan;Duan, Feiyu;Xiao, Mengqi;Li, Chong;Tang, Liying;Wei, Xiaoying;Jia, Jiqin;Yu, Weidong;Jiang, Weibin;Hoffmann, Ary A.;Cao, Lijun;Zhu, Jianqing;Liu, Qunxiu;Hong, Yang;Hong, Yang;Yu, Weidong
关键词:Paederus fuscipes; Genome assembly; Detoxification; PPE; Immune; Mutualistic interactions
-
A neutralizing monoclonal antibody-based blocking ELISA to detect bovine herpesvirus 1 and vaccination efficacy
作者:Liu, Wenxiao;Hong, Jiabing;Duan, Jinglong;Jiang, Bo;Zhu, Runan;Cheng, Jing;Li, Yongqing;Liu, Wenxiao;Duan, Jinglong;Jiang, Bo;Cheng, Jing;Li, Yongqing;Hong, Jiabing;Wang, Ping;Zhu, Runan
关键词:Bovine herpesvirus 1; Neutralizing monoclonal antibody; Blocking ELISA; Serological surveillance
-
Comprehensive Analysis of the Longissimus Dorsi Transcriptome and Metabolome Reveals the Regulatory Mechanism of Different Varieties of Meat Quality
作者:Wang, Zhengwen;Jiao, Ting;Wang, Zhengwen;Jiao, Ting;An, Xuejiao;Yang, Yonghui;Zhang, Lingyun;Zhao, Shengguo
关键词:cattle; meat quality; transcriptome; metabolome
-
Genome-Wide Identification of SnRK1 Catalytic alpha Subunit and FLZ Proteins in Glycyrrhiza inflata Bat. Highlights Their Potential Roles in Licorice Growth and Abiotic Stress Responses
作者:Yang, Chao;Shi, Guangyu;Li, Yuping;Luo, Ming;Wang, Ying;Li, Yongqing;Yang, Chao;Shi, Guangyu;Li, Yuping;Luo, Ming;Wang, Ying;Li, Yongqing;Yang, Chao;Shi, Guangyu;Li, Yuping;Luo, Ming;Wang, Ying;Li, Yongqing;Wang, Hongxia;Wang, Jihua;Yuan, Ling;Wang, Ying
关键词:SnRK1; FLZ; Glycyrrhiza inflata; stress response; growth
-
Identification and Characterization of chCR2, a Protein That Binds Chicken Complement Component 3d
作者:Jin, Huan;Jiang, Bo;Tu, Min;Xu, Jian;Cheng, Jing;Liu, Wenxiao;Zhang, Zhenhua;Li, Yongqing;Kong, ZiMeng;Jiang, Bo;Xu, Jian;Cheng, Jing;Liu, Wenxiao;Li, Yongqing
关键词:
-
Bioactive Functions of Lipids in the Milk Fat Globule Membrane: A Comprehensive Review
作者:Pan, Junyu;Chen, Meiqing;Zheng, Nan;Zhao, Shengguo;Zhang, Yangdong;Pan, Junyu;Li, Ning;Han, Rongwei;Yang, Yongxin
关键词:milk fat globule membrane; phospholipids; ganglioside; cholesterol; bioactive function
-
Whole-genome resequencing to unveil genetic characteristics and selection signatures of specific pathogen-free ducks
作者:Li, Lanlan;Gao, Caixia;Liu, Hongyi;Yu, Haibo;Chen, Hongyan;Xia, Changyou;Li, Lanlan;Quan, Jinqiang;Zhao, Shengguo
关键词:laboratory ducks; whole-genome resequencing; genetic characteristics; selection signature; single nucleo-tide polymorphisms