NAC transcription factor SlNOR-like1 plays a dual regulatory role in tomato fruit cuticle formation
文献类型: 外文期刊
第一作者: Liu, Gang-Shuai
作者: Liu, Gang-Shuai;Ji, Xiang;Peng, Zhen-Zhen;Li, Hong-Li;Niu, Xiao-Lin;Jia, Wen;He, Jian-Lin;Xiang, Lan-Ting;Qu, Gui-Qin;Zhu, Hong-Liang;Zhu, Ben-Zhong;Luo, Yun-Bo;Fu, Da-Qi;Huang, Hua;Huang, Hua;Grierson, Donald;Gao, Ying;Grierson, Donald;Gao, Ying;Gao, Hai-Yan
作者机构:
关键词: Cuticle; cutin; SlNOR-like1; tomato fruit; transcription factor; wax
期刊名称:JOURNAL OF EXPERIMENTAL BOTANY ( 影响因子:6.9; 五年影响因子:8.0 )
ISSN: 0022-0957
年卷期: 2023 年
页码:
收录情况: SCI
摘要: The plant cuticle is an important protective barrier on the plant surface, constructed mainly by polymerized cutin matrix and a complex wax mixture. Although the pathway of plant cuticle biosynthesis has been clarified, knowledge of the transcriptional regulation network underlying fruit cuticle formation remains limited. In the present work, we discovered that tomato fruits of the NAC transcription factor SlNOR-like1 knockout mutants (nor-like1) produced by CRISPR/Cas9 [clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9] displayed reduced cutin deposition and cuticle thickness, with a microcracking phenotype, while wax accumulation was promoted. Further research revealed that SlNOR-like1 promotes cutin deposition by binding to the promoters of glycerol-3-phosphate acyltransferase6 (SlGPAT6; a key gene for cutin monomer formation) and CUTIN DEFICIENT2 (SlCD2; a positive regulator of cutin production) to activate their expression. Meanwhile, SlNOR-like1 inhibits wax accumulation, acting as a transcriptional repressor by targeting wax biosynthesis, and transport-related genes 3-ketoacyl-CoA synthase1 (SlKCS1), ECERIFERUM 1-2 (SlCER1-2), SlWAX2, and glycosylphosphatidylinositol-anchored lipid transfer protein 1-like (SlLTPG1-like). In conclusion, SlNOR-like1 executes a dual regulatory effect on tomato fruit cuticle development. Our results provide a new model for the transcriptional regulation of fruit cuticle formation. In tomato fruit, SlNOR-like1 promotes cutin deposition by targeting and activating SlGPAT6 and SlCD2 . Meanwhile, SlNOR-like1 inhibits wax accumulation by targeting and inhibiting SlKCS1 , SlCER1-2 , SlWAX2 , and SlLTPG1-like .
分类号:
- 相关文献
作者其他论文 更多>>
-
Transcriptome combined with long non-coding RNA analysis reveals the potential molecular mechanism of high-CO2 treatment in delaying postharvest strawberry fruit ripening and senescence
作者:Liu, Gang-Shuai;Zhou, Jia-Hua;Chang, Hong;Wang, Bao-Gang;Wang, Yun-Xiang;Liu, Gang-Shuai;Fu, Da-Qi;Zhong, Chuan-Fei;Zong, Jing
关键词:Strawberry fruit; Ripening and senescence; High-CO 2 treatment; Transcriptome; Long non -coding RNA
-
Telomere-to-telomere genome assembly of bitter melon (Momordica charantia L. var. abbreviata Ser.) reveals fruit development, composition and ripening genetic characteristics
作者:Fu, Anzhen;Zheng, Yanyan;Wang, Qing;Zuo, Jinhua;Fu, Anzhen;Liu, Ye;Li, Jian;Guo, Jing;Grierson, Donald;Wen, Changlong;Zhang, Xuewen;Yu, Ying;Ma, Hong
关键词:
-
Long-term anthropogenic field management enhanced wheat productivity and resource utilization in the Loess Plateau region of China
作者:Wu, Peng;Wu, Qi;Huang, Hua;Xue, Jianfu;Sun, Min;Gao, Zhiqiang;Zhao, Gang;Li, Shangzhong;Wang, Lei;Cheng, Wanli;Fan, Tinglu;Zhang, Peng;Fu, Jian;Yang, Kejun;Bangura, Kemoh;Gao, Zhiqiang
关键词:Organic fertilizer; Soil fertility; Straw incorporation; Water and nitrogen utilization; Wheat productivity
-
Long noncoding RNA ARTA controls ABA response through MYB7 nuclear trafficking in Arabidopsis
作者:Yang, Jun;He, Reqing;Jiang, Liyun;Zhu, Youlin;Wang, Dong;Qu, Zhipeng;Adelson, David L.;Gu, Jinbao;Wang, Zhen-Yu;Zhan, Xiangqiang;Gao, Ying;Li, Sisi
关键词:
-
Multi-valent mRNA vaccines against monkeypox enveloped or mature viron surface antigens demonstrate robust immune response and neutralizing activity
作者:Zhang, Niubing;Cheng, Xiang;Li, Xuan;Zhu, Yilong;Li, Xiao;Mo, Ouyang;Yu, Huiqing;Zhu, Liqi;Zhang, Juan;Kuang, Linlin;Gao, Ying;Liang, Xiaozhen;Wang, Haikun;Hao, Pei;Cao, Ruiyuan;Li, Song;Zhong, Wu;Zhu, Yilong;Zhang, Niubing;Li, Honglin;Zhang, Niubing;Li, Honglin;Cheng, Xiang;Mo, Ouyang;Zhu, Liqi;Zhang, Juan;Gao, Ying;Liang, Xiaozhen;Wang, Haikun;Li, Xuan;Hao, Pei
关键词:monkeypox virus; enveloped and mature viron; multi-valent mRNA vaccines; immune response; neutralizing antibody
-
DNA methylation mediated by RdDM pathway and demethylation affects furanone accumulation through regulation of QUINONE OXIDOREDUCTASE in strawberry
作者:Li, Yunduan;Shi, Yanna;Li, Yichen;Lu, Jiao;Sun, Yunfan;Zhang, Yuanyuan;Chen, Wenbo;Grierson, Donald;Chen, Kunsong;Shi, Yanna;Chen, Wenbo;Chen, Kunsong;Shi, Yanna;Chen, Wenbo;Chen, Kunsong;Yang, Xiaofang;Jiang, Guihua;Grierson, Donald;Lang, Zhaobo;Lang, Zhaobo
关键词:
-
CRISPR/Cas9-mediated mutations of FANTASTIC FOUR gene family for creating early flowering mutants in tomato
作者:Shang, Lele;Tao, Jinbao;Song, Jianwen;Wang, Yaru;Zhang, Xingyu;Ge, Pingfei;Li, Fangman;Dong, Haiqiang;Gai, Wenxian;Ye, Zhibiao;Zhang, Yuyang;Shang, Lele;Ye, Zhibiao;Zhang, Yuyang;Grierson, Donald;Zhang, Yuyang;Zhang, Yuyang
关键词:CRISPR/Cas9; early flowering; FANTASTIC FOUR; germplasms; tomato