Visual and Super-Sensitive Detection of Maize Chlorotic Mottle Virus by Dot-ELISA and Au Nanoparticle-Based Immunochromatographic Test Strip
文献类型: 外文期刊
第一作者: Zhang, Cui
作者: Zhang, Cui;Guo, Mengmeng;Dong, Jinxi;Zhou, Xueping;Wu, Jianxiang;Dong, Jinxi;Wu, Jianxiang;Liu, Li;Zhou, Xueping
作者机构:
关键词: maize chlorotic mottle virus; monoclonal antibody; Dot-ELISA; immunochromatographic test strip; RT-PCR
期刊名称:VIRUSES-BASEL ( 影响因子:4.7; 五年影响因子:4.8 )
ISSN:
年卷期: 2023 年 15 卷 7 期
页码:
收录情况: SCI
摘要: Maize chlorotic mottle virus (MCMV) is the only species in the Mahromovirus genus and is often co-infected with one or several viruses of the Potyvirus genus, posing a great threat to the global maize industry. Effective viral integrated management measures are dependent on the timely and proper detection of the causal agent of the disease. In this work, six super-sensitive and specific monoclonal antibodies (mAbs) against MCMV were first prepared using purified MCMV virions as the immunogen. Then, the Dot enzyme-linked immunosorbent assay (Dot-ELISA) was established based on the obtained mAbs, and it can detect MCMV in infected maize leaf crude extracts diluted up to 1:10,240-fold (w/v, g/mL). Furthermore, a rapid and user-friendly Au nanoparticle-based immunochromatographic test strip (AuNP-ICTS) based on paired mAbs 7B12 and 17C4 was created for monitoring MCMV in point-of-care tests, and it can detect the virus in a 25,600-fold dilution (w/v, g/mL) of MCMV-infected maize leaf crude extracts. The whole test process for ICTS was completed in 10 min. Compared with conventional reverse transcription-polymerase chain reaction (RT-PCR), the detection endpoint of both serological methods is higher than that of RT-PCR, especially the Dot-ELISA, which is 12.1 times more sensitive than that of RT-PCR. In addition, the detection results of 20 blinded maize samples by the two serological assays were consistent with those of RT-PCR. Therefore, the newly created Dot-ELISA and AuNP-ICTS exhibit favorable application potential for the detection of MCMV in plant samples.
分类号:
- 相关文献
作者其他论文 更多>>
-
Inhibition mechanism of trans-resveratrol on thermally induced trans fatty acids in peanut oil
作者:Li, Tian;Guo, Qin;Qu, Yang;Liu, Hongzhi;Liu, Li;Wang, Qiang;Zhang, Yu
关键词:Mechanism; Trans -resveratrol; Peanut oil; Trans fatty acids
-
Effects of nitrogen limitation on Leymus Chinensis clonal growth form and bud bank
作者:Zuo, Shining;Zhou, Chengyang;Wang, Xiaonan;Huang, Ding;Liu, Li;Huang, Ding;Liu, Li
关键词:Nitrogen stress; Clonal plants; Bud type trade-off; Allocation; Leymus chinensis; Clonal growth form
-
Molecular Variation and Genomic Function of Citrus Vein Enation Virus
作者:Dou, Runqiu;Huang, Qingqing;Hu, Tao;Yu, Fengzhe;Hu, Hongxia;Wang, Yaqin;Zhou, Xueping;Qian, Yajuan;Zhou, Xueping
关键词:citrus vein enation virus; P0 protein; phase separation; hypersensitive response; suppressor
-
Facile fabrication of naphthalene-functionalized magnetic nanoparticles for efficient extraction of polycyclic aromatic hydrocarbons from environmental water and fish samples
作者:Peng, Xitian;Liu, Li;Hu, Xizhou;Yan, Wei;Zheng, Dan;Xia, Zhenzhen;Zhou, Youxiang;Xia, Hong;Peng, Lijun;Yu, Qiongwei
关键词:Polycyclic aromatic hydrocarbons; Magnetic solid-phase extraction; Naphthalene modified magnetic nanoparticles; Fish; GC-MS; MS
-
Genome editing of a dominant resistance gene for broad-spectrum resistance to bacterial diseases in rice without growth penalty
作者:Wang, Meixia;Zhou, Xueping;Zhou, Huanbin;Wang, Meixia;Zhou, Changyong;Wang, Meixia;Zhou, Huanbin;Li, Shaofang;Li, Huayang;Song, Congfeng;Xie, Wenya;Zuo, Shimin;Zhou, Xueping;Ji, Zhiyuan
关键词:Xa23; CRISPR/Cas9; TAL effector; rice
-
Characteristic Profile of the Hazardous, Nutritional, and Taste-Contributing Compounds during the Growth of Argopecten irradians with Different Shell Colors
作者:Wang, Teng;Peng, Jixing;Zhao, Xinnan;Lin, Yichen;Song, Dongru;Zhao, Yanfang;Jiang, Yanhua;Wu, Haiyan;Geng, Qianqian;Zheng, Guanchao;Guo, Mengmeng;Tan, Zhijun;Tan, Zhijun
关键词:bay scallop; shell color; growth; nutrient composition; hazard
-
Cell cycle-dependent kinase inhibitor GhKRP6, a direct target of GhBES1.4, participates in BR regulation of cell expansion in cotton
作者:Gu, Yu;Gu, Yu;Zhang, Jie;Liu, Le;Qanmber, Ghulam;Xing, Kun;Lu, Lili;Ma, Shuya;Li, Fuguang;Yang, Zuoren;Zhang, Jie;Qanmber, Ghulam;Liu, Zhao;Li, Fuguang;Liu, Li;Yang, Zuoren;Yang, Zuoren
关键词:cotton; KRP; cell cycle; cell expansion; cell wall; MAPK; GhCDKG