文献类型： 外文期刊
第一作者： Chen, Jingchao
作者： Chen, Jingchao;Cui, Hailan;Li, Zhiling;Yu, Haiyan;Li, Xiangju;Hou, Qiang
作者机构：
关键词： mutation; affinity; metabolomics; fitness; glyphosate; Eleusine indica
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN：
年卷期： 2023 年 24 卷 9 期
页码：
收录情况： SCI
摘要： Gene mutation is a basic evolutionary mechanism in plants under selection pressure of herbicides. Such mutation has pleiotropic effects on plant growth. We systemically investigated the effects of Pro106Leu (P106L), Pro106Ser (P106S), and Thr102Ile + Pro106Ser (TIPS) mutations on EPSPS functionality and fitness traits in Eleusine indica at the biochemical and physiological levels. The affinity of natural EPSPS for glyphosate was 53.8 times higher than that for phosphoenolpyruvate (PEP), as revealed by the dissociation constant; the constant decreased in both the P106L (39.9-fold) and P106S (46.9-fold) mutants but increased in the TIPS (87.5-fold) mutant. The Km (PEP) values of the P106L, P106S, and TIPS mutants were 2.4-, 0.7-, and 4.1-fold higher than that of natural EPSPS, corresponding to resistance levels of 2.5, 1.9, and 11.4, respectively. The catalytic efficiency values (maximum reaction rates) were 0.89-, 0.94-, and 0.26-fold higher than that of natural EPSPS. The levels of metabolites related to amino acids and nucleotides were significantly reduced in the mutated plants. The fitness costs were substantial for the biomass, total leaf area, seed number, and seedling emergence throughout the growth period in the plants with P106L and TIPS mutations. These results provide insights into EPSPS kinetics and their effect on plant growth.
分类号：
- 相关文献
作者其他论文 更多>>
-
Insights into mechanism of 1-hydroxy-2-naphthoic acid accumulation during phenanthrene degradation by Stutzerimonas frequens SL-6
作者：Guo, Liwei;Zhang, Xinyu;Hou, Qiang;Zhao, Baisuo;Wang, Haisheng;Wei, Hailei;Gu, Yilin;Zhang, Xinyu
关键词：Stutzerimonas frequens; Phenanthrene; Oxygenated PAHs; 1-Hydroxy-2-naphthoic acid
-
Affecting of Glyphosate Tolerance and Metabolite Content in Transgenic Arabidopsis thaliana Overexpressing EPSPS Gene from Eleusine indica
作者：Chen, Jingchao;Li, Zhiling;Yu, Haiyan;Cui, Hailan;Li, Xiangju
关键词：
Arabidopsis thaliana ; glyphosate; transgenic plant; secondary metabolites; targeted metabolome -
A New Pro-197-Ile Mutation in Amaranthus palmeri Associated with Acetolactate Synthase-Inhibiting Herbicide Resistance
作者：Ji, Meijing;Yu, Haiyan;Cui, Hailan;Chen, Jingchao;Li, Xiangju;Yu, Jialin
关键词：
Amaranthus palmeri ; resistance; imazethapyr;ALS gene mutation;ALS gene copy number;ALS gene expression -
Enhancing the synthesis efficiency of galacto-oligosaccharides of a β-galactosidase from Paenibacillus barengoltzii by engineering the active and distal sites
作者：Yu, Haiyan;Wang, Yulu;Ying, Jiabao;Liu, Bolin;Mohamed, Abeer;Wei, Xue;Yang, Yuji;Xin, Fengjiao;Yang, Zhisen;Liu, Xin;Sun, Linfeng;Guan, Feifei;Miao, Miao;Jiang, Zhengqiang;Yang, Shaoqing;Wang, Yulu;Xin, Fengjiao
关键词：beta-Galactosidase; GOS synthesis; Cryo-EM structure; Machine learning; Catalytic mechanism
-
Preliminary Investigation on Resistance of Beckmannia syzigachne to Clodinafop-Propargyl and Mesosulfuron-Methyl from China
作者：Peng, Licun;Li, Xiangju;Guo, Xiaotong;Li, Zheng;Chen, Jingchao;Wei, Shouhui;Cui, Hailan;Zhang, Shuai;Guo, Xiaotong
关键词：
Beckmannia syzigachne ; clodinafop-propargyl; mesosulfuron-methyl; resistance; mutation -
Increase in IAA levels by EPSPS copy number variation relates to fitness advantage in Eleusine indica
作者：Chen, Jingchao;Cui, Hailan;Li, Zhiling;Yu, Haiyan;Li, Xiangju;Yu, Qin
关键词：fitness advantage; glyphosate;
EPSPS copy number variation; RNA-seq; targeted metabolome; auxin -
Improving the herbicidal activity of mesosulfuron-methyl oil dispersion against Aegilops tauschii by decreasing particle size and adding adjuvant polyvinyl alcohol
作者：Yu, Haiyan;Wei, Cunyu;Ma, Xinle;Chen, Jingchao;Cui, Hailan;Li, Xiangju;Zhang, Tao;Liu, Feng;Wei, Cunyu;Yang, Juan;Ma, Xinle
关键词：herbicidal activity; formulation; particle size; rainfastness; selectiveness
