Single-Cell RNA Sequencing Reveals Atlas of Yak Testis Cells

文献类型： 外文期刊

第一作者： Wang, Xingdong

作者： Wang, Xingdong;Pei, Jie;Xiong, Lin;Guo, Shaoke;Cao, Mengli;Kang, Yandong;Ding, Ziqiang;La, Yongfu;Liang, Chunnian;Yan, Ping;Guo, Xian;Wang, Xingdong;Pei, Jie;Xiong, Lin;Guo, Shaoke;Cao, Mengli;Kang, Yandong;Ding, Ziqiang;La, Yongfu;Liang, Chunnian;Yan, Ping;Guo, Xian

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关键词： scRNA-seq; spermatogenesis; testis; germ cell; somatic cell; yak

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2023 年 24 卷 9 期

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收录情况： SCI

摘要： Spermatogenesis is a complex process that involves proliferation and differentiation of diploid male germ cells into haploid flagellated sperm and requires intricate interactions between testicular somatic cells and germ cells. The cellular heterogeneity of this process presents a challenge in analyzing the different cell types at various developmental stages. Single-cell RNA sequencing (scRNA-seq) provides a useful tool for exploring cellular heterogeneity. In this study, we performed a comprehensive and unbiased single-cell transcriptomic study of spermatogenesis in sexually mature 4-year-old yak using 10x Genomics scRNA-seq. Our scRNA-seq analysis identified six somatic cell types and various germ cells, including spermatogonial stem cells, spermatogonia, early-spermatocytes, late-spermatocytes, and spermatids in yak testis. Pseudo-timing analysis showed that Leydig and myoid cells originated from common progenitor cells in yaks. Moreover, functional enrichment analysis demonstrated that the top expressed genes in yak testicular somatic cells were significantly enriched in the cAMP signaling pathway, PI3K-Akt signaling pathway, MAPK signaling pathway, and ECM receptor interactions. Throughout the spermatogenesis process, genes related to spermatogenesis, cell differentiation, DNA binding, and ATP binding were expressed. Using immunohistochemical techniques, we identified candidate marker genes for spermatogonial stem cells and Sertoli cells. Our research provides new insights into yak spermatogenesis and the development of various types of cells in the testis, and presents more reliable marker proteins for in vitro culture and identification of yak spermatogonial stem cells in the later stage.

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