Identification of Novel Chromosomal Aberrations Induced by Co-60-gamma-Irradiation in Wheat-Dasypyrum villosum Lines
文献类型: 外文期刊
作者: Zhang, Jie 2 ; Jiang, Yun 1 ; Guo, Yuanlin 1 ; Li, Guangrong 2 ; Yang, Zujun 2 ; Xu, Delin 3 ; Xuan, Pu 1 ;
作者机构: 1.Sichuan Acad Agr Sci, Inst Biotechnol & Nucl Technol Res, Chengdu 610061, Sichuan, Peoples R China
2.Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Peoples R China
3.Zunyi Med Univ, Dept Cell Biol & Genet, Zunyi 563000, Guizhou, Peoples R China
4.Sichuan Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Chengdu 610066, Sichuan, Peoples R China
关键词: irradiation;wheat;Dasypyrum villosum;chromosomal aberrations;ND-FISH
期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.923; 五年影响因子:6.132 )
ISSN: 1422-0067
年卷期: 2015 年 16 卷 12 期
页码:
收录情况: SCI
摘要: Mutations induced by radiation are widely used for developing new varieties of plants. To better understand the frequency and pattern of irradiation-induced chromosomal rearrangements, we irradiated the dry seeds of Chinese Spring (CS)-Dasypyrum villosum nullisomic-tetrasomic (6A/6D) addition (6V) line (2n = 44), WD14, with Co-60--rays at dosages of 100, 200, and 300 Gy. The M-0 and M-1 generations were analyzed using Feulgen staining and non-denaturing fluorescence in situ hybridization (ND-FISH) by using oligonucleotide probes. Abnormal mitotic behavior and chromosomes with structural changes were observed in the M-0 plants. In all, 39 M-1 plants had structurally changed chromosomes, with the B genome showing the highest frequency of aberrations and tendency to recombine with chromosomes of the D genome. In addition, 19 M-1 plants showed a variation in chromosome number. The frequency of chromosome loss was considerably higher for 6D than for the alien chromosome 6V, indicating that 6D is less stable after irradiation. Our findings suggested that the newly obtained -induced genetic materials might be beneficial for future wheat breeding programs and functional gene analyses.
- 相关文献
作者其他论文 更多>>
-
The positive effects of Camellia oleifera oil on lifespan in Caenorhabditis elegans
作者:Liu, Li;Zhou, Jiasi;Kong, Qingbo;Zhang, Jie;Wang, Heng;Xiang, Zhuoya;Chen, Tao;Zhou, Lijun;Feng, Shiling;Yang, Hongyu;Huang, Yan;Ding, Chunbang;Xu, Zhou;Xiang, Zhuoya
关键词:Camellia oleifera oil; Caenorhabditis elegans; Lifespan; Transcriptomics; Metabolomics
-
Whole genome doubling-induced the enrichment of H3K27me3 in genes carrying specific TEs in Aegilops tauschii
作者:Guo, Hongwei;Zhang, Guoyan;Zhou, Min;Wan, Min;Zhu, Bo;Zeng, Deying;Zeng, Zixian;Zhang, Guoyan;Zhu, Bo;Zeng, Deying;Zeng, Zixian;Yang, Zujun
关键词:whole-genome doubling; Aegilops tauschii; transcriptome; transposable elements
-
Genes and pathways correlated with heat stress responses and heat tolerance in maize kernels
作者:Chen, Yan;Zhang, Jie;Fu, Junjie;Li, Huihui;Chen, Yan;Chen, Shoukun;Du, Tingting;Li, Huihui;Yang, Qin
关键词:maize kernel; heat response; heat tolerance; genes; pathways
-
Glucosinolates or erucic acid, which one contributes more to volatile flavor of fragrant rapeseed oil?
作者:Liang, Qiang;Xiong, Wei;Xu, Xia;Zhao, Ling;Xuan, Pu;Yao, Yingzheng;Liang, Qiang;Xiong, Wei;Xuan, Pu;Yao, Yingzheng;Zhou, Qi;Zhou, Qi;Cui, Cheng
关键词:Fragrant rapeseed oil; Erucic acid; Glucosinolate; Silicon dioxide degumming; Key odorants
-
Development of a Set of Wheat-Rye Derivative Lines from Hexaploid Triticale with Complex Chromosomal Rearrangements to Improve Disease Resistance, Agronomic and Quality Traits of Wheat
作者:Wang, Tingting;Li, Guangrong;Jiang, Chengzhi;Zhou, Yuwei;Yang, Zujun;Yang, Ennian;Li, Jianbo;Zhang, Peng;Dundas, Ian
关键词:FISH; rye; wheat; chromosome rearrangement; disease resistance
-
Development and characterization of wheat-Aegilops kotschyi 1U(k)(1A) substitution line with positive dough quality parameters
作者:JIANG, Yun;WANG, De-li;HAO, Ming;LIU, Deng-cai;JIANG, Yun;ZHANG, Jie;JIANG, Yun;WANG, De-li;HAO, Ming;LIU, Deng-cai
关键词:Aegilops kotschyi; common wheat; 1U(k)(1A) substitution line; HMW-GS; processing quality
-
Development and Characterization of Near-Isogenic Lines Derived from Synthetic Wheat Revealing the 2 kb Insertion in the PPD-D1 Gene Responsible for Heading Delay and Grain Number Improvement
作者:Ning, Shunzong;Li, Shengke;Zhang, Lianquan;Liu, Dengcai;Ning, Shunzong;Li, Shengke;Xu, Kai;Liu, Dongmei;Ma, Li;Ma, Chunfang;Hao, Ming;Zhang, Lianquan;Huang, Lin;Chen, Xuejiao;Jiang, Bo;Yuan, Zhongwei;Liu, Dengcai;Chen, Wenjie;Zhang, Bo;Jiang, Yun
关键词:synthetic wheat; photoperiod-1; number of days to heading; grain number per spike; spike length; spikelet number per spike