Study on the Hyperglycemic Effect of GLP-1 in Spinibarbus denticulatus by Oral Administration and Intraperitoneal Injection Methods
文献类型: 外文期刊
作者: Luo, Wei 1 ; Li, Luojia 1 ; Zhang, Yue 1 ; Xu, Zhou 2 ; Xiong, Yinlin 3 ; Guo, Zhonggang 4 ; Zhang, Ning 1 ; Zhang, Yibo 1 ; Chen, Pengyu 1 ; Wang, Yan 1 ; Du, Zongjun 1 ;
作者机构: 1.Sichuan Agr Univ, Coll Anim Sci & Technol, Chengdu, Sichuan, Peoples R China
2.Mianyang Acad Agr Sci, Mianyang, Sichuan, Peoples R China
3.Original Stock Farm Leiocassis longirostris Sichua, Chongzhou, Sichuan, Peoples R China
4.Agr & Rural Bur Chongzhou, Chongzhou, Sichuan, Peoples R China
期刊名称:AQUACULTURE NUTRITION ( 影响因子:3.5; 五年影响因子:3.6 )
ISSN: 1353-5773
年卷期: 2023 年 2023 卷
页码:
收录情况: SCI
摘要: Glucagon-like peptide-1 (GLP-1), one of the expression products of the proglucagon (pg) gene, is an incretin mainly secreted by the gastrointestinal system. In mammals, GLP-1 has hypoglycemic and food-inhibiting effects; while in some fish species, it has been confirmed to increase blood glucose by promoting gluconeogenesis and stimulating glycogenolysis. In order to more deeply understand the role of GLP-1 in the process of glycometabolism in herbivorous fish, the pg gene was cloned from Spinibarbus denticulatus to obtain its sequence characteristics, and the changes in blood glucose level and pg gene expression in S. denticulatus were further explored by feeding with three kinds of carbohydrates and intraperitoneal injection of GLP-1. Basal and temporal blood glucose levels and pg gene expression of S. denticulatus (91.68 +/- 10.79 g) were measured at 0, 1, 3, 5, 7, and 12 h after oral administration (n=4). Then, the changes of blood glucose levels and pg and glucokinase (gk) gene expressions of S. denticulatus (94.29 +/- 10.82 g) were determined at 0, 30, 60, and 120 min after intraperitoneal injection (n=4). It was shown that polysaccharides could induce the upregulation of pg gene expression faster than monosaccharides and stimulate the secretion of GLP-1 in the intestine. Intraperitoneal injection of GLP-1 peptide rapidly raised blood glucose levels, and pg gene expression in the anterior intestine, whole brain, and hepatopancreas decreased continuously after 30 minutes. These results showed that S. denticulatus might inhibit the excessive accumulation of blood glucose by reducing the expression of the pg gene and increasing the expression of gk gene in a short time. It was speculated that GLP-1 of S. denticulatus might have a "gut-brain-liver" pathway similar to mammals in glycemia regulation. Therefore, this study provided a novel perspective for explaining the functional differences of GLP-1 in herbivorous fish and mammals.
- 相关文献
作者其他论文 更多>>
-
The background adaptation of the skin color in the loach Paramisgurnus dabryanus
作者:Luo, Wei;Zhang, Shoudong;Zhang, Xiaoyang;Zhang, Yibo;Chen, Pengyu;Jiang, Jun;Yang, Shiyong;Yang, Song;Du, Zongjun;Wang, Tianzhu;Guo, Zhonggang;Xiong, Yinlin;Xu, Zhou
关键词:Background adaptation; Skin color; Discoloration mechanism; Paramisgurnus dabryanus
-
The positive effects of Camellia oleifera oil on lifespan in Caenorhabditis elegans
作者:Liu, Li;Zhou, Jiasi;Kong, Qingbo;Zhang, Jie;Wang, Heng;Xiang, Zhuoya;Chen, Tao;Zhou, Lijun;Feng, Shiling;Yang, Hongyu;Huang, Yan;Ding, Chunbang;Xu, Zhou;Xiang, Zhuoya
关键词:Camellia oleifera oil; Caenorhabditis elegans; Lifespan; Transcriptomics; Metabolomics
-
Integrated single-molecule real-time sequencing and RNA sequencing reveal the molecular mechanisms of salt tolerance in a novel synthesized polyploid genetic bridge between maize and its wild relatives
作者:Li, Xiaofeng;Wang, Xingyu;Ma, Qiangqiang;Zhong, Yunfeng;Zhang, Yibo;Li, Yingzheng;He, Ruyu;Zhou, Yang;He, Jianmei;Iqbal, Muhammad Zafar;Rong, Tingzhao;Tang, Qilin;Zhang, Ping;Li, Yang;Cheng, Mingjun;Yan, Xu;Li, Yan
关键词:SMRT-sequencing; RNA-sequencing; Zea mays; Tripsacum dactyloides; Zea perennis; Genetic bridge; Salt tolerance
-
The complete mitochondrial genome of Rhinogobius maculagenys (Gobiidae:Gobionellinae)
作者:Hu, Jia;Li, Yan-hua;Liu, Jun;Xu, Zhou;Wu, Hai-Bing;Cheng, Chao;Chu, Xu-Dong;Wu, Zhang-Dong
关键词:Mitogenome; Rhinogobius maculagenys; phylogenetic analysis; Rhinogobius
-
Ethylene Signaling Pathway Genes in Strawberry and Their Expression Patterns during Fruit Ripening
作者:Zhang, Yunting;Deng, Meiyi;Guo, Chenhui;Chen, Yan;Lin, Yuanxiu;Chen, Qing;Wang, Yan;Zhang, Yong;Luo, Ya;Wang, Xiaorong;Tang, Haoru;Gu, Xianjie
关键词:strawberry; FaETRs; FaEIN3; EINs; fruit ripening
-
Light Quality and Sucrose-Regulated Detached Ripening of Strawberry with Possible Involvement of Abscisic Acid and Auxin Signaling
作者:Jiang, Leiyu;Chen, Xinpeng;Deng, Meiyi;Li, Xiaotong;Zhou, Aiyang;Suo, Mengyue;Gao, Weiliang;Lin, Yuanxiu;Wang, Yan;He, Wen;Li, Mengyao;Chen, Qing;Zhang, Yong;Luo, Ya;Wang, Xiaorong;Tang, Haoru;Zhang, Yunting;Gu, Xianjie;Lin, Yuanxiu;Wang, Yan;He, Wen;Wang, Xiaorong;Tang, Haoru;Zhang, Yunting
关键词:detached ripening; strawberry; light quality; sucrose
-
Resveratrol Improves the Digestive Ability and the Intestinal Health of Siberian Sturgeon
作者:Yang, Shiyong;Xu, Wenqiang;Feng, Langkun;Zhang, Chaoyang;Yan, Chaozhan;Zhang, Jiajin;Yan, Taiming;He, Zhi;Du, Zongjun;Luo, Wei;Huang, Xiaoli;Lai, Jiansheng;Du, Xiaogang;Wu, Jiayun;Li, Yunkun
关键词:resveratrol; Siberian sturgeon; digestive ability; microbiome