Integrated analysis of small RNAs, transcriptome and degradome sequencing reveal the drought stress network in Agropyron mongolicum Keng
文献类型： 外文期刊
作者： Fan, Bobo 1 ; Sun, Fengcheng 2 ; Yu, Zhuo 1 ; Zhang, Xuefeng 1 ; Yu, Xiaoxia 1 ; Wu, Jing 1 ; Yan, Xiuxiu 1 ; Zhao, Yan 3 ; Nie, Lizhen 2 ; Fang, Yongyu 2 ; Ma, Yanhong 1 ;
作者机构： 1.Inner Mongolia Agr Univ, Agr Coll, Hohhot, Peoples R China
2.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot, Peoples R China
3.Inner Mongolia Agr Univ, Coll Grassland Resources & Environm, Hohhot, Peoples R China
关键词： Agropyron mongolicum Keng; drought resistance; microRNAs; transcriptome; degradome; integration analysis; co-expression network
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）
ISSN： 1664-462X
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： Agropyron mongolicum (A. mongolicum) is an excellent gramineous forage with extreme drought tolerance, which lives in arid and semiarid desert areas. However, the mechanism that underlies the response of microRNAs (miRNAs) and their targets in A. mongolicum to drought stress is not well understood. In this study, we analyzed the transcriptome, small RNAome (specifically the miRNAome) and degradome to generate a comprehensive resource that focused on identifying key regulatory miRNA-target circuits under drought stress. The most extended transcript in each collection is known as the UniGene, and a total of 41,792 UniGenes and 1,104 miRNAs were identified, and 99 differentially expressed miRNAs negatively regulated 1,474 differentially expressed target genes. Among them, eight miRNAs were unique to A. mongolicum, and there were 36 target genes. A weighted gene co-expression network analysis identified five hub genes. The miRNAs of five hub genes were screened with an integration analysis of the degradome and sRNAs, such as osa-miR444a-3p.2-MADS47, bdi-miR408-5p_1ss19TA-CCX1, tae-miR9774_L-2R-1_1ss11GT-carC, ata-miR169a-3p-PAO2, and bdi-miR528-p3_2ss15TG20CA-HOX24. The functional annotations revealed that they were involved in mediating the brassinosteroid signal pathway, transporting and exchanging sodium and potassium ions and regulating the oxidation-reduction process, hydrolase activity, plant response to water deprivation, abscisic acid (ABA) and the ABA-activated signaling pathway to regulate drought stress. Five hub genes were discovered, which could play central roles in the regulation of drought-responsive genes. These results show that the combined analysis of miRNA, the transcriptome and degradation group provides a useful platform to investigate the molecular mechanism of drought resistance in A. mongolicum and could provide new insights into the genetic engineering of Poaceae crops in the future.
- 相关文献
作者其他论文 更多>>
-
Integrated metabolomics and transcriptomics reveals difference involved in flavonoid and indole alkaloids biosynthesis in potato tuber flesh
作者：Wu, Juan;Ma, Yanhong;Zhang, Zhicheng;Zhang, Sheng;Wu, Xiaojuan;Wang, Peijie;Xie, Rui;Lu, Chunxing;Zhang, Zhicheng;Wang, Dan;Ma, Yanhong
关键词：Colored potato tuber flesh; HybridsF1; Flavonoid; Indole alkaloids; Correlation analysis
-
Fungal communities are more sensitive to nitrogen fertilization than bacteria in different spatial structures of silage maize under short-term nitrogen fertilization
作者：Bai, Lanfang;Li, Bingzhen;Wang, Yufen;Lu, Zhanyuan;Zhang, Dejian;Fang, Jing;Zhang, Xiangqian;Sun, Fengcheng;Zhao, Xiaoqing;Lu, Zhanyuan;Bai, Lanfang;Zhang, Xiangqian;Zhao, Xiaoqing;Lu, Zhanyuan;Fang, Jing
关键词：Silage maize; Nitrogen fertilizers; Microbial composition; High-throughput sequencing; Spatial structure
-
Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers (Solanum tuberosum)
作者：Wu, Xiaojuan;Ma, Yanhong;Wu, Juan;Wang, Peijie;Zhang, Zhicheng;Fan, Bobo;Liu, Xuting;Zhang, Zhicheng;Xie, Rui;Nie, Li Zhen;Liu, Jie;Wei, Wei
关键词：anthocyanin metabolism; gene expression; high-throughput sequencing; miRNA target; miRNAs; purple potatoes
-
Fermentation weight loss, fermentation quality, and bacterial community of ensiling of sweet sorghum with lactic acid bacteria at different silo densities
作者：Xu, Haiwen;Wu, Nier;Na, Na;Sun, Lin;Zhao, Yi;Ding, Haijun;Fang, Yongyu;Xue, Yanlin;Wang, Tianwei;Zhong, Jin
关键词：bacterial community; bacterial diversity; fermentation weight loss; microbial counts; nutritional compositions; organic acid; sweet sorghum silage
-
Improving evaluation of potato resistance to Rhizoctonia solani infection by optimizing inoculum-based method combined with toxin-based assay
作者：Zhang, Xiaoyu;Huo, Hongli;Xing, Xing;Li, Dezhou;Yu, Zhuo;Li, Dezhou;Huo, Hongli;Lian, Yong;Hao, Jianjun
关键词：Potato; Rhizoctonia solani AG2-1; Stem canker; Disease resistance
-
Characterization and comparison of the bacterial microbiota in different gastrointestinal tract compartments of Mongolian horses
作者：Su, Shaofeng;Zhao, Yiping;Liu, Guiqin;Du, Ming;Wu, Jing;Bai, Dongyi;Li, Bei;Bou, Gerelchimeg;Zhang, Xinzhuang;Dugarjaviin, Manglai;Su, Shaofeng;Liu, Zongzheng;Liu, Guiqin
关键词：16S rRNA V3-V4; high-throughput sequencing; intestinal microbiota; microbiome; Mongolian horses
-
Functional Analysis of Three miRNAs in Agropyron mongolicum Keng under Drought Stress
作者：Zhang, Xuting;Fan, Bobo;Yu, Zhuo;Yu, Xiaoxia;Lei, Xuefeng;Ma, Yanhong;Nie, Lizhen;Sun, Fengcheng;Zhao, Yan
关键词：Agropyron mongolicum Keng; drought-responsive microRNA; NEXT-generation sequencing; target gene prediction; functional analysis
