Establishment of a tilapia lake virus (TiLV)-susceptible cell line for anti-TiLV Chinese herbal compound screening
文献类型: 外文期刊
作者: Zhu, Song 1 ; Miao, Bo 1 ; Zhang, Yu-Zhou 1 ; Yang, Qin 1 ; Deng, Xun-Teng 4 ; Gao, Li-Hong 3 ; Zheng, Ji-Shu 3 ; Pu, De-Cheng 3 ; Wang, De-Shou 2 ; Su, Sheng-Qi 1 ;
作者机构: 1.Southwest Univ, Coll Fisheries, Chongqing 400715, Peoples R China
2.Southwest Univ, Sch Life Sci, Key Lab Aquat Sci Chongqing, Key Lab Freshwater Fish Reprod & Dev,Minist Educ, Chongqing 400715, Peoples R China
3.Chongqing Acad Agr Sci, Chongqing 401329, Peoples R China
4.Yongchuan Dist Chongqing Anim Husb & Fishery Ctr, Chongqing 402160, Peoples R China
关键词: Tilapia lake virus; Cell line; Chinese herbal compound; Tilapia
期刊名称:AQUACULTURE ( 影响因子:4.5; 五年影响因子:4.6 )
ISSN: 0044-8486
年卷期: 2023 年 577 卷
页码:
收录情况: SCI
摘要: Tilapia lake virus (TiLV) has caused many deadly epidemics in wild and farmed tilapia, posing a great threat to the global tilapia-based industry. It is necessary and urgent to develop effective measures for TiLV control. Previously, we isolated a TiLV strain from naturally diseased tilapia, and its main target tissues are liver, spleen and brain. In the study, a TiLV-susceptive cell line is established based on the tissue tropism, and anti-TiLV activity of 11 Chinese herbal compounds (CHCs) is checked in vitro. A cell line from the brain of tilapia, designated as Nt-B, can be continuously subcultured for >100 passages and maintain a good proliferating state in L-15 media supplemented with 10% FBS at 28 degrees C. Following infection with TiLV, a series of cytopathic effects can be observed, including rounding, accumulation, vacuolation and detachment of cells. Enveloped viral particles can be observed in TiLV-infected cells, and TiLV-specific bands are identified by PCR analysis, confirming susceptibility of Nt-B cells to TiLV. For anti-TiLV activity evaluation, the selected CHCs show inhibition activity on TiLV-induced CPEs in different degrees except CHC-I. Two kinds of CHCs, CHC-II and CHC-X, have inhibition activity on TiLV-induced CPEs higher than 80%. Besides, the 90% inhibitory concentrations of CHC-II and CHC-X on TiLV are 51.12 +/- 3.04 and 68.28 +/- 6.37 mg/L, respectively. In summary, the newly established Nt-B cell line provides a useful tool for TiLV-related studies, moreover, CHC-II is a promising therapeutic agent against TiLV infection. Further studies will be required to reveal the active compounds and anti-TiLV mechanism of CHC-II.
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Identification and pathogenetic study of tilapia lake virus (TiLV) isolated from naturally diseased tilapia
作者:He, Tao;Zhang, Yu-Zhou;Miao, Bo;Su, Sheng-Qi;Zhu, Song;Zhang, Qing-Qing;Wang, De-Shou;Zhu, Song;Gao, Li-Hong;Zheng, Ji-Shu;Pu, De-Cheng;Zeng, Wei-Wei
关键词:Tilapia lake virus; Tilapia; Virus detection and isolation; Koch's postulates; Tissue tropism