文献类型： 外文期刊

作者： Liu, Sheng-Rong ² ; Ke, Bin-Rong ¹ ; Zhang, Wei-Rui ² ; Liu, Xin-Rui ¹ ; Wu, Xiao-Ping ¹ ;

作者机构： 1.Fujian Agr & Forestry Univ, Mycol Res Ctr, Fuzhou 350002, Peoples R China

2.Ningde Normal Univ, Dept Biol, Ningde 352100, Peoples R China

3.Fujian Acad Agr Sci, Inst Edible Fungi, Fuzhou 35003, Peoples R China

关键词： Ganoderma lucidum;Monokaryon;Mating;Polysaccharide;Triterpene

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The aim of this study was to breed G. lucidum strains simultaneously rich in polysaccharides and triter penes by mating of basidiospore monokaryons of two commercial cultivars, G. lucidum JC and AL. By incubation of freshly collected basidiospores, 31 monokaryons were isolated (19 from strain JC, and 12 from strain AC), and showed significant differences in mycelial growth and contents of polysaccharide and triterpene. Mating between the two strains yielded 83 instances of sexual compatibility. Of the hybrids isolated, sixteen ones that showed moderate and fast substrate colonization were evaluated in terms of fruiting. All the tested hybrids produced fruiting bodies but varied considerably in time to first harvest, productivity, yield distribution, and pileus shape and color. Significant differences in polysaccharide content (from 6.79 to 18.21 mg/g dry weight) and triterpene content (from 5.90 to 10.87 mg/g dry weight) were also observed among the hybrids. Notably, high concentrations of polysaccharides (16.63 mg/g dry weight) and triterpenes (10.50 mg/g dry weight) were both present in hybrid H-23, with satisfactory biological efficiency (19.41%). An antagonism test and random amplified polymorphic DNA (RAPD) analysis indicated that H-23 is a new hybrid strain. This hybrid may be suitable for use in commercial production of G. lucidum for manufacture of more effective health-promoting and medicinal products. To our knowledge, the present study is the first report on the development of new G. lucidum strains via mating by means of basidiospore-derived monokaryons. (C) 2016 Elsevier B.V. All rights reserved.