Blood Metabolites and Faecal Microbial Communities in Nonpregnant and Early Gestation Ewes in Highly Cold Areas
文献类型: 外文期刊
作者: Wu, Zhiwu 1 ; Yang, Yanyan 3 ; Wang, Biao 3 ; Gebeyew, Kefyalew 1 ; Tang, Shaoxun 1 ; Han, Xuefeng 1 ; He, Zhixiong 1 ; Tan, Zhiliang 1 ;
作者机构: 1.Chinese Acad Sci, Natl Engn Lab Pollut Control & Waste Utilizat Live, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Inst Subtrop Agr,CAS Key Lab Agroecol Proc Subtrop, Changsha 410125, Peoples R China
2.Univ Chinese Acad Sci, Beijing 101408, Peoples R China
3.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010031, Peoples R China
关键词: early gestation; pregnancy; metabolite; faecal microbiota; biomarker
期刊名称:BIOLOGY-BASEL ( 影响因子:4.2; 五年影响因子:4.4 )
ISSN:
年卷期: 2023 年 12 卷 11 期
页码:
收录情况: SCI
摘要: Simple Summary In this study, we elucidated the changes in metabolites and microbial communities in pregnant ewes during early gestation, and tried to find new biomarkers to predict early pregnancy in sheep. Significant differences were found in the blood biochemical parameters and blood metabolites, but not in the faecal microbial community. At the same time, five potential pregnancy biomarkers were found to predict early pregnancy in ewes. These detected differences will provide a scientific theoretical basis for the rational nutritional regulation of farmers, and these newly discovered biomarkers will promote the development of technology for detecting early pregnancy in sheep in highly cold areas.Abstract Ewes undergo complex metabolic changes during pregnancy. Understanding the specific process of these changes is a necessary prerequisite in ewes for regulating and intervening in order to maintain pregnancies. However, there have been relatively few studies on the specific changes that occur in nutritional metabolism in pregnant ewes during early gestation, especially for some landrace ewes in highly cold areas. Therefore, this study aimed to (1) elucidate the changes in metabolites and microbial communities in pregnant ewes during early gestation using metabolomics and 16S ribosomal RNA gene (rDNA) amplicon sequencing approaches, and to (2) discover novel early pregnancy-induced biomarkers in the blood and faeces. Rams were placed together with ewes on D0 and removed on D45. During early gestation, blood and faecal samples were collected from ewes in a highly cold area for analysing the metabolites and microbial communities; these were retrospectively classified as the early gestation pregnant (EP) ewe group or the nonpregnant (NP) ewe group based on the lambing status recorded during the expected delivery period. The differences in the plasma biochemical parameters, plasma metabolites, and faecal microbial communities of pregnant and nonpregnant ewes were characterised. The GC, IL-6, O-acetyl-l-serine, L-glutamine, and 6-acetamido-2-oxohexanoic acid were screened out as potential biomarkers for evaluating the occurrence of early pregnancy. These novel early pregnancy-induced metabolites discovered in ewes might allow for the development of technologies to detect early pregnancies in sheep in highly cold areas.
- 相关文献
作者其他论文 更多>>
-
The ERβ-cAMP signaling pathway regulates estradiol-induced ovine oocyte meiotic arrest
作者:Yang, Bingxue;Zhao, Yufen;Yu, Kai;Weng, Yu;Du, Chenguang;Li, Haijun;Yang, Bingxue;Zhao, Yufen;Yu, Kai;Weng, Yu;Du, Chenguang;Li, Haijun;An, Yang;Yang, Yanyan;Yu, Boyang
关键词:Sheep; Cumulus-oocyte complex; 17 beta-estradiol; Cyclic adenosine monophosphate; Estrogen nuclear receptor
-
Study of spermatogenic and Sertoli cells in the Hu sheep testes at different developmental stages
作者:Su, Jie;Su, Jie;Zhao, Feifei;Zhang, Yue;Su, Hong;Li, Kuo;Cao, Guifang;Yang, Yanyan;Wang, Daqing;Song, Yongli;Song, Yongli;Cao, Guifang
关键词:blood-testes barrier; Hu sheep; Sertoli cell; spermatocytes; spermatogonia; testes
-
An integrated transcriptome and microbial community analysis reveals potential mechanisms for increased immune responses when replacing silybum marianum meal with soybean meal in growing lambs
作者:Zhang, Tianxi;Ren, Yanbo;Yang, Chao;Gebeyew, Kefyalew;He, Zhixiong;Tan, Zhiliang;Zhang, Tianxi;Yang, Chao;Gebeyew, Kefyalew;He, Zhixiong;Tan, Zhiliang;Ren, Yanbo;Gao, Min
关键词:silybum marianum meal; soybean meal; lambs; cytokines; microbial community; transcriptome
-
Glutathione and selenium nanoparticles have a synergistic protective effect during cryopreservation of bull semen
作者:Li, Shubin;Zhang, Wenqi;Su, Liya;Liu, Gang;Ren, Jingyu;Dai, Yanfeng;Wang, Biao;Ma, Yuzhen
关键词:SeNPs; GSH; oxidative stress; bull; semen cryopreservation
-
Glutathione ameliorates the meiotic defects of copper exposed ovine oocytes via inhibiting the mitochondrial dysfunctions
作者:Ren, Jingyu;Li, Liping;Liu, Yongbin;Dai, Yanfeng;Wang, Biao;Li, Shubin;Ma, Yuzhen;Su, Liya;Liu, Gang
关键词:Glutathione; Copper; Meiotic defects; Mitochondrial dysfunctions
-
Estimates of Genomic Heritability and the Marker-Derived Gene for Re(Production) Traits in Xinggao Sheep
作者:Liu, Zaixia;Shi, Caixia;Dai, Lingli;Chai, Yuan;Zhang, Wenguang;Liu, Zaixia;Dai, Lingli;Zhang, Wenguang;Fu, Shaoyin;Wang, Biao;Liu, Xuewen;Dai, Lingli;Liu, Yongbin
关键词:Xinggao sheep; LS; BWT; WWT; ADG; genetic parameter evaluation; MATs
-
Effects of dietary oat supplementation on carcass traits, muscle metabolites, amino acid profiles, and its association with meat quality of Small-tail Han sheep
作者:Wang, Li-wei;Su, Shao-feng;He, Xiao-long;Fu, Shao-yin;Wang, Biao;Wang, Da-qing;Chen, Xin;Terigele;Li, Xiao-dong;Jiang, Li-li;He, Jiang-feng;Yun, Na-na;Liu, Yong-bin;Wang, Yun-fei;Zhao, Jie;Belobrajdic, Damien P.
关键词:Meat quality; Metabolomics; Flavor; Small -tail Han sheep; Oat(Avena sativa L; )