文献类型: 外文期刊
作者: Zhao, Yue 1 ; Chen, Mingming 1 ; Li, Yao 1 ; Xu, Xueling 1 ; Wu, Sujun 1 ; Liu, Zhimei 1 ; Qi, Shiyu 1 ; Yi, Guang 1 ; Zhang, Xiaosheng 2 ; Zhang, Jinlong 2 ; Guo, Xiaofei 2 ; Yu, Kun 1 ; Deng, Shoulong 1 ; Li, Yan 1 ; Lian, Zhengxing 1 ;
作者机构: 1.China Agr Univ, Coll Anim Sci & Technol, Beijing Key Lab Anim Genet Improvement, Natl Engn Lab Anim Breeding,Key Lab Anim Genet &, Beijing 100193, Peoples R China
2.Tianjin Acad Agr Sci, Inst Anim Husb & Vet Med, Tianjin 300381, Peoples R China
3.Chinese Acad Med Sci, NHC Key Lab Human Dis Comparat Med, Inst Lab Anim Sci, Beijing 100021, Peoples R China
4.Peking Union Med Coll, Comparat Med Ctr, Beijing 100021, Peoples R China
关键词: meat; MSTN; FGF5; sheep; 90-day feeding; rat
期刊名称:LIFE-BASEL ( 影响因子:3.251; )
ISSN:
年卷期: 2022 年 12 卷 2 期
页码:
收录情况: SCI
摘要: MSTN and FGF5 gene knockout sheep generated by the CRISPR/Cas9 system exhibit the 'double-muscle' phenotype, and increased density and length of hairs, providing valuable new breeding material. In a previous study, we obtained MSTN and FGF5 double-knockout sheep of significant breeding value. In this study, we carried out a 90-day feeding study in Wistar rats to assess the safety of genome-edited mutton. Seven rat groups with 10 females and 10 males per group were fed different concentrations (3.75%, 7.5%, and 15%) of double-knockout mutton or wild-type mutton in a conventional commercial diet for 90 days. At the end of the feeding, routine urine and blood tests and measurements of blood biochemical indicators were performed. Furthermore, the major organs of each group of rats were weighed and examined histopathologically. Although there were significant differences among the groups in some parameters, all values were within the normal ranges. Therefore, the 90-day rat feeding study showed that the meat from MSTN and FGF5 double-knockout sheep did not have any long-term adverse effects on rat health. This study also provides valuable reference information for assessing the safety of meat from animals with knockout of multiple genes.
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