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A novel method of domestication combined with ARTP to improve the reduction ability of Bacillus velezensis to Cr(VI)

文献类型： 外文期刊

作者： Bao, Zhijie 1 ; Wang, Xiaomei 2 ; Wang, Qiangfeng 3 ; Zou, Liang 1 ; Peng, Lianxin 1 ; Li, Lijiao 1 ; Tu, Wenying 1 ; Li, Qiang 1 ;

作者机构： 1.Chengdu Univ, Sichuan Engn & Technol Res Ctr Coarse Cereal Indus, Sch Food & Biol Engn, Key Lab Coarse Cereal Proc,Minist Agr & Rural Affa, Chengdu, Sichuan, Peoples R China

2.Chinese Acad Sci, Chengdu Inst Biol, CAS Key Lab Environm & Appl Microbiol, Environm Microbiol Key Lab Sichuan Prov, Chengdu, Sichuan, Peoples R China

3.Sichuan Acad Agr Sci, Biotechnol & Nucl Technol Res Inst, Chengdu, Sichuan, Peoples R China

4.2025 Chengluo Ave, Chengdu, Sichuan, Peoples R China

关键词： Genetic engineering; ARTP; Bioremediation; Environmental engineering; Pollution control

期刊名称：JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING （ 影响因子：7.7； 五年影响因子：7.3 ）

ISSN： 2213-2929

年卷期： 2023 年 11 卷 1 期

页码：

收录情况： SCI

摘要： In this study, the Cr(VI)-resistant bacterium LYB-23 was isolated from waste chromium residues in Sichuan and identified as Bacillus velezensis. The chromium tolerance of LYB-23 was improved by domestication (continuously increasing the chromium concentration in the medium from 60 to 200 mg/L). Subsequently, we screened a mutant strain, B. velezensis SA-23, using atmospheric pressure room temperature plasma (ARTP) mutagenesis for 90 s based on the domesticated strain. The minimum inhibitory concentration of B. velezensis on chromium increased from 80 mg/L for the original strain to 200 mg/L for the domesticated strain and to 400 mg/L for the mutant strain. The Cr(VI) reduction experiment results show that SA-23 can completely reduce 100 mg/L Cr(VI) within 24 h, and the reduction rate of 200 mg/L Cr(VI) reached 78.45% within 72 h. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) analysis showed that ARTP mutagenesis did not change the type of functional groups on the surface of bacteria after reductive adsorption of Cr(VI) and the crystalline state of Cr(III); however, mutagenesis changed the biological morphology of bacteria and greatly enhanced the role of phosphate groups in the process of adsorption and reduction of Cr(VI). This experiment for the first time used the combined domestication and ARTP mutagenesis to effectively improve the tolerance of bacteria to Cr(VI) and their reduction adsorption ef-ficiency, which proved to be an efficient method for the breeding of environmental remediation engineering bacteria and for promoting the application of microbial remediation.

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