Transcriptome profiling of Microbacterium resistens MZT7 reveals mechanisms of 17?-estradiol response and biotransformation
文献类型： 外文期刊
作者： Hao, Peng 1 ; Lv, Zongshuo 1 ; Wu, Sicheng 1 ; Zhang, Xiqing 1 ; Gou, Changlong 2 ; Wang, Lixia 3 ; Zhu, Yanbin 4 ; Basang, Wangdui 4 ; Gao, Yunhang 1 ;
作者机构： 1.Jilin Agr Univ, Coll Vet Med, Changchun 130118, Peoples R China
2.Inner Mongolia Univ Natl, Coll Anim Sci & Technol, Tongliao 028000, Peoples R China
3.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Changchun 130102, Peoples R China
4.Tibet Acad Agr & Anim Husb Sci, Inst Anim Husb & Vet Med, Lhasa 850009, Peoples R China
关键词： 17?-estradiol; Microbacterium resistens MZT7; Microbial biodegradation; Intracellular enzymes; Transcriptome analysis; ABC transporters
期刊名称：ENVIRONMENTAL RESEARCH （ 影响因子：8.3； 五年影响因子：8.2 ）
ISSN： 0013-9351
年卷期： 2023 年 217 卷
页码：
收录情况： SCI
摘要： 17 beta-estradiol (E2) pollution has attracted much attention, and the existence of E2 poses certain risks to the environment and human health. However, the mechanism of microbial degradation of E2 remains unclear. In this study, the location of E2-degrading enzymes was investigated, and transcriptome analysis of Microbacterium resistens MZT7 (M. resistens MZT7) exposed to E2. The degradation of E2 by M. resistens MZT7 was via the biological action of E2-induced intracellular enzymes. With the RNA sequencing, we found 1109 differentially expressed genes (DEGs). Among them, 773 genes were up-regulated and 336 genes were down-regulated. The results of the RNA sequencing indicated the DEGs were related to transport, metabolism, and stress response. Genes for transport, transmembrane transport, oxidoreductase activity, ATPase activity, transporter activity and quorum sensing were up-regulated. Genes for the tricarboxylic acid cycle, ribosome, oxidative phosphorylation and carbon metabolism were down-regulated. In addition, heterologous expression of one enzymes efficiently degraded E2. These findings provide some new insights into the molecular mechanism of biotransformation of E2 by M. resistens MZT7.
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