文献类型： 外文期刊

作者： Li, Weibin ¹ ; Wang, Zedong ¹ ; Wang, Xinwei ¹ ; Cui, Li ² ; Huang, Wenyuan ³ ; Zhu, Zhaoyong ⁴ ; Liu, Zhenjiang ¹ ;

作者机构： 1.Jiangsu Univ, Inst Environm & Ecol, Sch Environm & Safety Engn, Zhenjiang 212013, Peoples R China

2.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China

3.Guizhou Acad Agr Sci, Inst Plant Protect, Guiyang 550006, Peoples R China

4.Zhenjiang D&A Biol Technol Co Ltd, Zhenjiang 212009, Peoples R China

关键词： Lateral flow immunoassay; Deoxynivalenol and zearalenone; Nanozyme; Smartphone; Aqueous environment

期刊名称：JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING （ 影响因子：7.7； 五年影响因子：7.3 ）

ISSN： 2213-2929

年卷期： 2023 年 11 卷 5 期

页码：

收录情况： SCI

摘要： Deoxynivalenol (DON) and zearalenone (ZEN) pose a serious threat to human health, and have been frequently detected in the aqueous environment. To protect consumers from the harm of mycotoxins, a nanozyme-mediated multiplexed lateral flow immunoassay (LFIA) integrated with a smartphone was developed for rapid, highly sensitive and simultaneous quantitative detection of DON and ZEN in the aqueous environment. Highly efficient peroxidase mimicking core-shell Au@Pt nanozymes were synthesized by one-pot method, and then used as signal amplification to highly improve sensitivity of the detection, while a smartphone-based quantitative detection device could rapidly quantify results to improve the detection efficiency of the LIFA for on-site detection. After optimization, the detection time of the assay was 10 min, and the detection limits of the LIFA for DON/ZEN were 0.24/0.04 ng/mL, which were improved 416 and 150 folds compared to the conventional gold nanoparticles (GNPs)-based LFIA. Moreover, there was no obvious cross-reaction with other related mycotoxins, indicating that LFIA had a high specificity. The average recoveries of DON and ZEN from corn, wheat and three water samples were obtained from 94.3 % to 107.9 % with relative standard deviations of 0.2-7.6 %. Furthermore, the accuracy and reliability of the LIFA were evaluated with three spiked water samples, and the results presented good correlations with analytic results from the enzyme-linked immunosorbent assay (R2 =0.988 for DON, and 0.983 for ZEN). The results indicate the proposed LIFA was potentially a rapid, on-site simultaneous and highly sensitive method for DON and ZEN detection in the aqueous environment.