Biochemical characterization of a key laccase-like multicopper oxidase of artificially cultivable Morchella importuna provides insights into plant-litter decomposition
文献类型: 外文期刊
作者: Zhang, Qiang 2 ; Miao, Renyun 2 ; Liu, Tianhai 2 ; Huang, Zhongqian 2 ; Peng, Weihong 2 ; Gan, Bingcheng 2 ; Zhang, Xi 1 ;
作者机构: 1.Sichuan Agr Univ, Coll Resources, Chengdu, Sichuan, Peoples R China
2.Sichuan Acad Agr Sci, Soil & Fertilizer Inst, Natl Local Joint Engn Lab Breeding & Cultivat Edi, Chengdu, Sichuan, Peoples R China
3.Minist Agr, Sci Observing & Expt Stn Agromicrobial Resource &, Chengdu, Sichuan, Peoples R China
关键词: Morchella importuna SCYDJ1-A1; AA1_3 family; Laccase-like multicopper oxidase; Transcript level; Overexpression; Biochemical characterization
期刊名称:3 BIOTECH ( 影响因子:2.406; 五年影响因子:3.203 )
ISSN: 2190-572X
年卷期: 2019 年 9 卷 5 期
页码:
收录情况: SCI
摘要: The aim of this study is to determine the key laccase-encoding gene in the life cycle of Morchella importuna SCYDJ1-A1, and to characterize the biochemical properties of the laccase. Two laccase-like multicopper oxidase (LMCO) genes were identified in the genome of M. importuna SCYDJ1-A1 as putative laccase-encoding genes. The two genes, belonging to Auxiliary Activity family 1 subfamily 3, were named as MiLacA and MiLacB. Phylogenetic analysis of deduced amino acid sequences showed that MiLacA is closest to a LMCO of M. importuna 22J1, while MiLacB had low similarity with known Morchella LMCOs. Real-time quantitative PCR results showed that MiLacA was expressed at much higher levels than MiLacB throughout the entire course of artificial cultivation. MiLacA was overexpressed in Pichia pastoris as a recombinant protein. Biochemical characterization of the purified enzyme showed that MiLacA simultaneously possessed laccase and polyphenol-oxidase activities. MiLacA could be strongly inhibited by Fe2+, which is unusual. The optimum pH was four and optimum temperature was 60 degrees C. The enzyme retained over 74% of the laccase activity after 16-h incubation at 60 degrees C, which means that its thermostability is at the forefront among the currently known laccases. Our findings may help to elucidate how the laccase of M. importuna is involved in decaying lignin in plant litter, and could also provide a candidate thermostable laccase for potential industrial application.
- 相关文献
作者其他论文 更多>>
-
Integrated multi-omic analyses on yellow Flammulina filiformis cultivar reveal postharvest oxidative damage responses
作者:Fu, Yu;Tan, Hao;Wang, Bo;Peng, Weihong;Yu, Yang;Fu, Yu;Sun, Qun;Tan, Hao
关键词:Flammulina filiformis; Genome sequencing; Transcriptome; Proteomics; Oxidative stress
-
Development of Multiple Nucleotide Polymorphism Molecular Markers for Enoki Mushroom (Flammulina filiformis) Cultivars Identification
作者:Liu, Fei;Wang, Shi-Hui;Zhao, Rui-Lin;Wang, Shi-Hui;Jia, Ding-Hong;Tan, Hao;Wang, Bo;Zhao, Rui-Lin
关键词:mushroom; MNP marker; sequencing; cultivar
-
Mechanism of Inhibiting the Growth and Aflatoxin B-1 Biosynthesis of Aspergillus flavus by Phenyllactic Acid
作者:Zhao, Chi;Penttinen, Petri;Zhang, Lingzi;Zhang, Xiaoping;Zhao, Chi;Dong, Ling;Zhang, Fengju;Li, Zhihua;Penttinen, Petri
关键词:phenyllactic acid; Aspergillus flavus; aflatoxin B-1; transcriptome; metabolome
-
Black Truffles Affect Quercus aliena Physiology and Root-Associated nirK- and nirS-Type Denitrifying Bacterial Communities in the Initial Stage of Inoculation
作者:Kang, Zongjing;Li, Yan;Zhang, Xiaoping;Penttinen, Petri;Gu, Yunfu;Kang, Zongjing;Li, Xiaolin;Ye, Lei;Zhang, Bo;Zhang, Xiaoping;Penttinen, Petri
关键词:Tuber indicum; Tuber melanosporum; Quercus aliena; ectomycorrhizosphere; denitrifying bacteria; growth of host plants; soil properties
-
Screening and characterization of a salt-tolerant aflatoxin B-1-degrading strain isolated from Doubanjiang, a Chinese typical red pepper paste
作者:Zhao, Chi;Dong, Ling;Zhang, Fengju;Yang, Zebo;LI, Zhihua;Zhao, Chi;Zhang, Xiaoping;Luo, Yongliang
关键词:aflatoxin B-1 (AFB(1)); biodegradation; salt tolerance; Bacillus velezensis AD8
-
Large-scale commercial cultivation of morels: current state and perspectives
作者:Xu, Yingyin;Tang, Jie;Wang, Yong;He, Xiaolan;Tan, Hao;Yu, Yang;Chen, Ying;Peng, Weihong;Xu, Yingyin;Tang, Jie;Wang, Yong;He, Xiaolan;Tan, Hao;Yu, Yang;Chen, Ying;Peng, Weihong;Xu, Yingyin;Tang, Jie;Wang, Yong;He, Xiaolan;Tan, Hao;Yu, Yang;Chen, Ying;Peng, Weihong;Xu, Yingyin;Tang, Jie;Wang, Yong;He, Xiaolan;Tan, Hao;Yu, Yang;Chen, Ying;Peng, Weihong
关键词:Morel mushrooms; Commercial cultivation; Key technology; Unstable yield
-
Impacts of size reduction and alkaline-soaking pretreatments on microbial community and organic matter decomposition during wheat straw composting
作者:Tan, Hao;Chen, Jian;Tan, Hao;Yu, Yang;Liu, Tianhai;Miao, Renyun;Hu, Rongping;Peng, Weihong;Zhu, Yongqun;Miao, Renyun;Miao, Renyun
关键词:Size reduction; Alkaline soaking; Wheat-straw composting; Microbial community shift; Enzymatic activity