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Actinobacteria associated with Chinaberry tree are diverse and show antimicrobial activity

文献类型: 外文期刊

作者: Li, Jing 1 ; Shen, Meiling 1 ; Chen, Qiang 1 ; Ao, Xiaolin 1 ; Liao, Decong 1 ; Gu, Yunfu 1 ; Xu, Kaiwei 1 ; Ma, Menggen 1 ; Yu, Xiumei 1 ; Xiang, Quanju 1 ; Chen, Ji 1 ; Zhang, Xiaoping 1 ; Liu, Maoke 2 ; Penttinen, Petri 3 ; Penttinen, Petri 4 ;

作者机构: 1.Sichuan Agr Univ, Coll Resource & Environm Sci, Dept Microbiol, Yaan 625000, Peoples R China

2.Sichuan Acad Agr Sci, Rice & Sorghum Res Inst, Biotechnol Ctr, Luzhou 646100, Peoples R China

3.Zhejiang Agr & Forestry Univ, Sch Environm & Resource Sci, Zhejiang Prov Key Lab Carbon Cycling Forest Ecosy, Linan 311300, Peoples R China

4.Univ Helsinki, Ecosyst & Environm Res Programme, FIN-00014 Helsinki, Finland

期刊名称: SCIENTIFIC REPORTS

ISSN: 2045-2322

年卷期: 2018 年 8 卷

页码:

收录情况: SCI

摘要: Many actinobacteria produce secondary metabolites that include antimicrobial compounds. Since most of the actinobacteria cannot be cultivated, their antimicrobial potential awaits to be revealed. We hypothesized that the actinobacterial endophyte communities inside Melia toosendan (Chinaberry) tree are diverse, include strains with antimicrobial activity, and that antimicrobial activity can be detected using a cultivation independent approach and co-occurrence analysis. We isolated and identified actinobacteria from Chinaberry, tested their antimicrobial activities, and characterized the communities using amplicon sequencing and denaturing gradient gel electrophoresis as cultivation independent methods. Most of the isolates were identified as Streptomyces spp., whereas based on amplicon sequencing the most abundant OTU was assigned to Rhodococcus, and Tomitella was the most diverse genus. Out of the 135 isolates, 113 inhibited the growth of at least one indicator organism. Six out of the 7577 operational taxonomic units (OTUs) matched 46 cultivated isolates. Only three OTUs, Streptomyces OTU4, OTU11, and OTU26, and their corresponding isolate groups were available for comparing co-occurrences and antimicrobial activity. Streptomyces OTU4 correlated negatively with a high number of OTUs, and the isolates corresponding to Streptomyces OTU4 had high antimicrobial activity. However, for the other two OTUs and their corresponding isolate groups there was no clear relation between the numbers of negative correlations and antimicrobial activity. Thus, the applicability of co-occurrence analysis in detecting antimicrobially active actinobacteria could not be proven.

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