Molecular characterization and overexpression of mnp6 and vp3 from Pleurotus ostreatus revealed their involvement in biodegradation of cotton stalk lignin
文献类型： 外文期刊
作者： Wang, Yan 1 ; Li, Guoqing 1 ; Jiao, Xiaoyu 1 ; Cheng, Xi 1 ; Abdullah, Muhammad 1 ; Li, Dahui 1 ; Lin, Yi 1 ; Cai, Yongpi 1 ;
作者机构： 1.Anhui Agr Univ, Sch Life Sci, Hefei 230036, Anhui, Peoples R China
2.Anhui Acad Agr Sci, Hort Res Inst, Hefei 230031, Anhui, Peoples R China
关键词： Pleurotus ostreatus; Class II peroxidase; Bioinformatics; Overexpression; Lignin degradation
期刊名称：BIOLOGY OPEN （ 影响因子：2.422； 五年影响因子：2.647 ）
ISSN： 2046-6390
年卷期： 2019 年 8 卷 2 期
页码：
收录情况： SCI
摘要： Fungal secretory heme peroxidase (Class II POD) plays a significant role in biomass conversion due to its lignin-degrading activity. In this study, genome-wide identification and bioinformatics were performed to analyze Pleurotus ostreatus peroxidases (PoPODs). A total of six manganese peroxidases (MnPs) and three versatile peroxidases (VPs) were obtained. Bioinformatics analysis and qRT-PCR showed that P. ostreatus mnp6 (Pomnp6) and P. ostreatus vp3 (Povp3) could be involved in lignin degradation. Both Pomnp6 and Povp3 transgenetic fungi showed significantly increased lignin degradation of cotton stalks. H-1-NMR revealed that Pomnp6 and Povp3 may preferentially degrade S-lignin in cotton stalks and mainly break beta-O-4' bond linkages and hydroxyl. These results support the possible utility of Pomnp6 and Povp3 in natural straw resources and development of sustainable energy.
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