Precise identification of wheat - Thinopyrum intermedium translocation chromosomes carrying resistance to wheat stripe rust in line Z4 and its derived progenies
文献类型: 外文期刊
作者: Lang, Tao 1 ; La, Shixiao 1 ; Li, Bin 1 ; Yu, Zhihui 1 ; Chen, Qiheng 1 ; Li, Jianbo 1 ; Yang, Ennian 2 ; Li, Guangrong 1 ;
作者机构: 1.Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Sichuan, Peoples R China
2.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China
关键词: Thinopyrum intermedium; wheat; rust resistance; chromosome translocation
期刊名称:GENOME ( 影响因子:2.166; 五年影响因子:2.474 )
ISSN: 0831-2796
年卷期: 2018 年 61 卷 3 期
页码:
收录情况: SCI
摘要: The wheat - Thinopyrum intermedium derived line Z4 has displayed novel and effective stripe rust resistance for over 40 years. This study aimed to precisely identify the chromosome constitution of line Z4 and determine the stripe rust resistance contribution using multicolor fluorescent in situ hybridization (FISH) and molecular marker analysis. The results indicated that the Z4 line (2n = 44) contained two pairs of non-Robertsonian translocations without the 3A chromosomes of wheat. FISH karyotypes of F-3 progenies derived from crosses between Z4 and MY11 indicated that the transmission of the translocated chromosomes appeared normal and the number of wheat chromosomes 3A and 3D frequently varied. The FISH signal distribution of a new repetitive probe, named Oligo-3A1, confirmed the physical breakage points on chromosome 3AL incorporated into translocated chromosomes. PLUG markers revealed the breakage points on chromosomes 3A, 7J(S), and 3D invloved in the translocated chromosomes, and they were designated as T3DS-3AS.3AL-7J(S)S and T3AL-7J(S)S.7J(S)L. Stripe rust resistances surveys indicated that the proximal region of 7J(S)S or 7J(S)L may confer the resistance at the adult plant stage. The precise characterization of the chromosome complements of wheat - Th. intermedium Z4 and derived progenies has demonstrated the importance of combining cytogenetic and molecular approaches in the genomics era for further wheat genetic manipulation and breeding purposes.
- 相关文献
作者其他论文 更多>>
-
Whole genome doubling-induced the enrichment of H3K27me3 in genes carrying specific TEs in Aegilops tauschii
作者:Guo, Hongwei;Zhang, Guoyan;Zhou, Min;Wan, Min;Zhu, Bo;Zeng, Deying;Zeng, Zixian;Zhang, Guoyan;Zhu, Bo;Zeng, Deying;Zeng, Zixian;Yang, Zujun
关键词:whole-genome doubling; Aegilops tauschii; transcriptome; transposable elements
-
Development of a Set of Wheat-Rye Derivative Lines from Hexaploid Triticale with Complex Chromosomal Rearrangements to Improve Disease Resistance, Agronomic and Quality Traits of Wheat
作者:Wang, Tingting;Li, Guangrong;Jiang, Chengzhi;Zhou, Yuwei;Yang, Zujun;Yang, Ennian;Li, Jianbo;Zhang, Peng;Dundas, Ian
关键词:FISH; rye; wheat; chromosome rearrangement; disease resistance
-
Molecular and Cytogenetic Identification of Wheat-Thinopyrum intermedium Double Substitution Line-Derived Progenies for Stripe Rust Resistance
作者:Li, Guangrong;Chen, Qiheng;Jiang, Wenxi;Zhang, Ahui;Yang, Zujun;Yang, Ennian
关键词:chromosome translocation; ND-FISH; Oligo-FISH painting; rust resistance; Thinopyrum intermedium; wheat
-
A major yellow rust resistance QTL on chromosome 6A shows increased frequency in recent Norwegian spring wheat cultivars and breeding lines
作者:Lin, Min;Lillemo, Morten;Dieseth, Jon Arne;Alsheikh, Muath;Yang, Ennian;Holzapfel, Josef;Schuermann, Friederike;Morales, Laura;Michel, Sebastian;Buerstmayr, Hermann;Bhavani, Sridhar
关键词:
-
Molecular and cytogenetic dissection of stripe rust resistance gene Yr83 from rye 6R and generation of resistant germplasm in wheat breeding
作者:Li, Guangrong;Zhang, Yao;Yang, Zujun;Li, Jianbo;Zhang, Peng;Ma, Qiang;Yang, Ennian;Dundas, Ian
关键词:FISH; rye; wheat; stripe rust; Yr83
-
Location of Tandem Repeats on Wheat Chromosome 5B and the Breakpoint on the 5BS Arm in Wheat Translocation T7BS.7BL-5BS Using Single-Copy FISH Analysis
作者:Zhang, Wei;Tang, Zongxiang;Luo, Jie;Fu, Shulan;Li, Guangrong;Yang, Zujun;Yang, Manyu;Yang, Ennian
关键词:single-copy FISH; ND-FISH; tandem repeats; wheat translocation; breakpoint
-
Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization
作者:Yang, Manyu;Yang, Wuyun;Yang, Ennian;Yang, Manyu;Yang, Wuyun;Yang, Ennian;Yang, Manyu;Yang, Wuyun;Yang, Ennian;Yang, Zujun
关键词:wheat; ND-FISH; genetic diversity; chromosomal translocation