Purification and Identification of Novel Antioxidant Peptides from Hydrolysates of Peanuts (Arachis hypogaea) and Their Neuroprotective Activities
文献类型： 外文期刊
作者： Ma, Rui 1 ; Bai, Jinrong 1 ; Huang, Yuting 3 ; Wang, Zhiqiu 1 ; Xu, Yongju 3 ; Huang, Yan 1 ; Zhong, Kai 3 ; Huang, Yina 1 ; Gao, Hong 3 ; Bu, Qian 1 ;
作者机构： 1.Sichuan Univ, West China Hosp 4, Inst Syst Epidemiol, West China Sch Publ Hlth,Mol Toxicol Lab Sichuan P, Chengdu 610041, Peoples R China
2.Chengdu Univ Tradit Chinese Med, State Key Lab Southwestern Chinese Med Resources, Chengdu 611137, Peoples R China
3.Sichuan Univ, Coll Biomass & Engn & Hlth Food, Evaluat Res Ctr, Dept Food Sci & Technol, Chengdu 610065, Peoples R China
4.Sichuan Acad Agr Sci, Ind Crops Res Inst, Chengdu 610300, Peoples R China
关键词： Arachis hypogaea; peanut peptides; antioxidant activity; neuroprotection; PC12 cells
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2023 年 71 卷 15 期
页码：
收录情况： SCI
摘要： Peanut (Arachis hypogaea) peptides have various functional activities and a high utilization value. This study aims to isolate and characterize antioxidant peptides from peanut protein hydrolysates and further evaluate their neuroprotection against oxidative damage to PC12 cells induced by 6-hydroxydopamine (6-OHDA). After the peanut protein was hydrolyzed with pepsin and purified using ultrafiltration and gel chromatography, six peptides were identified and sequenced by high-performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Out of these six peptides, Pro-Gly-Cys-Pro-Ser-Thr (PGCPST) exhibited a desirable antioxidant capacity, as determined using the 1,1-diphenyl-2-picrylhydrazyl, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, and hydroxyl radical scavenging assays. Moreover, our results indicated that the peptide PGCPST effectively increased the cell viability and reduced the cell apoptosis in 6-OHDA-induced PC12. RNA sequencing further showed that the neuroprotective effect of the peptide PGCPST was mediated via sphingolipid metabolism-related pathways. With further research efforts, the peptide PGCPST was expected to develop into a new neuroprotective agent.
- 相关文献
作者其他论文 更多>>
-
Effects of solid-state fermentation with Bacillus subtilis Y4 on the quality of Yibin Yacai
作者：Zou, Yuke;Zhong, Yixin;Zhou, Qian;Chen, Qian;Wu, Yanping;Zhong, Kai;Gao, Hong;Jia, Zhenchao;Xu, Wei;Wei, Shugu
关键词：Yibin Yacai; Bacillus subtilis Y4; Solid-state fermentation; Flavor -related metabolites; Microbial communities
-
Effects of assorted radishes on the flavor development and bacterial community succession of radish paocai during fermentation
作者：Zheng, Zimeng;Zhou, Qian;Wu, Yanping;Zhong, Kai;Gao, Hong;Zhang, Xiaohong;Zhang, Fantao;Jia, Zhenchao
关键词：Varieties; Radish paocai; Bacterial community succession; Flavor profiles; Correlation analysis
-
The positive effects of Camellia oleifera oil on lifespan in Caenorhabditis elegans
作者：Liu, Li;Zhou, Jiasi;Kong, Qingbo;Zhang, Jie;Wang, Heng;Xiang, Zhuoya;Chen, Tao;Zhou, Lijun;Feng, Shiling;Yang, Hongyu;Huang, Yan;Ding, Chunbang;Xu, Zhou;Xiang, Zhuoya
关键词：Camellia oleifera oil; Caenorhabditis elegans; Lifespan; Transcriptomics; Metabolomics
-
Genetic diversity of field Fusarium asiaticum and Fusarium graminearum isolates increases the risk of fungicide resistance
作者：Zhang, Ya-Zhou;Li, Zhuo;Man, Jie;Xu, Dan;Wen, Lan;Yang, Chen;Xu, Qiang;Jiang, Qian-Tao;Chen, Guo-Yue;Deng, Mei;Qi, Peng-Fei;Wei, Yu-Ming;Zhang, Ya-Zhou;Li, Zhuo;Man, Jie;Xu, Dan;Wen, Lan;Yang, Chen;Xu, Qiang;Jiang, Qian-Tao;Chen, Guo-Yue;Deng, Mei;Qi, Peng-Fei;Wei, Yu-Ming;Peng, Yun-Liang;Zhong, Kai
关键词：Fusarium head blight; Virulence; Whole-genome sequencing; Genetic diversity; Fungicide
-
Improvement of physicochemical characteristics, flavor profiles and functional properties in Chinese radishes via spontaneous fermentation after drying
作者：Zheng, Zimeng;Zhou, Qian;Chen, Qian;Wu, Yanping;Zhong, Kai;Gao, Hong;Gao, Jia;Yang, Feng;Yang, Feng;Zhong, Kai
关键词：correlation analyses; flavor profiles; functional properties; radishes; spontaneous fermentation after drying; texture characteristics
-
A multifunctional chitosan-derived conformal coating for the preservation of passion fruit
作者：Zhou, Yifan;Zhong, Yixin;Li, Ling;Zhong, Kai;Yan, Bin;Jiang, Kai;Gao, Jia;Pan, Mingfei
关键词：Chitosan; Tannic acid; Coating; Preservation; Washability
-
A Comparison of Lipid Contents in Different Types of Peanut Cultivars Using UPLC-Q-TOF-MS-Based Lipidomic Study
作者：Huang, Yuting;Ma, Rui;Zhong, Kai;Gao, Hong;Xu, Yongju;Bu, Qian
关键词：peanuts; oleic acid; lipidomics; oxidation stability
