Pseudomonas composti isolate from oyster digestive tissue specifically binds with norovirus GII.6 via Psl extracellular polysaccharide
文献类型： 外文期刊
作者： Yu, Yongxin 1 ; Han, Feng 1 ; Yang, Mingshu 1 ; Zhang, Xiaoya 1 ; Chen, Yunfei 1 ; Yu, Mingxia 1 ; Wang, Yongjie 1 ;
作者机构： 1.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Minist Agr, Lab Qual & Safety Risk Assessment Aquat Prod Stora, Beijing, Peoples R China
4.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Minist Agr & Rural Affairs, Shanghai, Peoples R China
5.Hainan Trop Ocean Univ, Coll Food Sci & Engn, Sanya, Peoples R China
关键词： Norovirus; Binding; Pseudomonas composti; Exopolysaccharide; Oyster
期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY （ 影响因子：5.4； 五年影响因子：5.5 ）
ISSN： 0168-1605
年卷期： 2023 年 406 卷
页码：
收录情况： SCI
摘要： Oysters are recognized as important vectors for human norovirus transmission in the environment. Whether norovirus binds to bacteria in oyster digestive tissues (ODTs) remains unknown. To shed light on this concern, ODT-54 and ODT-32, positive for histo-blood group antigen (HBGA)-like substances, were isolated from ODTs and identified as Pseudomonas composti and Enterobacter cloacae, respectively. The binding of noroviruses (GII.4 and GII.6 P domains) to bacterial cells (ODT-32 and ODT-54; in situ assay) as well as extracted extracellular polysaccharides (EPSs; in vitro assay) was analyzed by flow cytometry, confocal laser scanning microscopy, ELISA, and gene knock-out mutants. ODT-32 bound to neither GII.4 nor GII.6 P domains, while ODT-54 spe-cifically binds with GII.6 P domain through Psl, an exopolysaccharide encoded by the polysaccharide synthesis locus (psl), identified based on gene annotation, gene transcription, Psl specific staining, and ELISAs. These findings attest that ODT bacteria specifically bind with certain norovirus genotypes in a strain-dependent manner, contributing to a better understanding of the transmission and enrichment of noroviruses in the environment.
- 相关文献
作者其他论文 更多>>
-
Survey of the biogenic amines in typical foods commonly consumed from the Chinese market
作者：Zhang, Xuan;Fang, Changling;Lou, Xiaoyi;Han, Feng;Li, Siman;Shi, Yongfu;Huang, Dongmei
关键词：Biogenic amines; Fish; Cheese; Alcoholic beverage; Risk assessment
-
Identification of 2,2',4,5,5' -Pentachlorobiphenyl (PCB101) metabolites and their transmission characteristics in silver crucian carp (Carassius auratus gibelio)
作者：Wang, Shuai;Wang, Meng-Yuan;Shi, Yong-Fu;Han, Feng;Ye, Hong-Li;Cai, You-Qiong;Wu, Di;Tian, Liang-Liang;Zhang, Xuan;Tang, Yun-Yu;Wang, Shuai;Wang, Meng-Yuan;Han, Feng;Wu, Di;Wang, Shuai
关键词：PCB101; Metabolite; Identification; Transfer characteristics; GC-MS/MS
-
A baseline study on the distribution characteristics and health risk assessment of cadmium in edible tissues of the swimming crabs (Portunus trituberculatus) from Shanghai, China
作者：Wu, Di;Shi, Yongfu;Wang, Mengyuan;Ran, Maoxia;Wang, Yuan;Tian, Liangliang;Ye, Hongli;Han, Feng;Wu, Di;Wang, Mengyuan;Ran, Maoxia
关键词：Cadmium; Portunus trituberculatus; Bioaccumulation; Health risk; East China Sea
-
Determination of 18 beta-Agonists in Aquatic Products Using High-Performance Liquid Chromatography Tandem Mass Spectrometry
作者：Han, Feng;Chen, Qing-ping;Yang, Guangxin;Tian, Liang-liang;Yu, Hui-juan;Shi, Yong-fu;Kong, Cong;Zhai, Wenlei;Fodjo, Essy Kouadio
关键词：beta-agonist; ractopamine; aquatic product; enzymolysis; fish; veterinary drug; banned drug
-
Effect of Cu Nanoparticles on the Properties of PP-g-PHMG/PE Monofilament and Its Antifouling Application
作者：Wang, Yue;Han, Feng;Shi, Jiangao;Shu, Aiyan;Wang, Yue;Shu, Aiyan;Zhang, Min;Yu, Wenwen
关键词：
-
Determination of Methylene Blue and Its Metabolite Residues in Aquatic Products by High-Performance Liquid Chromatography-Tandem Mass Spectrometry
作者：Zhang, Xuan;Hui, Yunhua;Fang, Changling;Wang, Yuan;Han, Feng;Lou, Xiaoyi;Cai, Youqiong;Kong, Cong;Zhang, Xuan;Fang, Changling;Han, Feng;Lou, Xiaoyi;Kong, Cong;Fodjo, Essy Kouadio
关键词：high-performance liquid chromatography-tandem mass spectrometry; methylene blue; disinfectant; aquatic products
-
An Effective and Efficient Sample Preparation Method for 2-Methyl-Isoborneol and Geosmin in Fish and Their Analysis by Gas Chromatography-Mass Spectrometry
作者：Tian, Liang-liang;Han, Feng;Huang, Xuan-Yun;Kong, Cong;Shi, Yong-Fu;Cai, You-Qiong;Samanidou, Victoria F.;Fodjo, Essy Kouadio;Zhai, Wenlei
关键词：
