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Detection of Blackleg Resistance Gene Rlm1 in Double-Low Rapeseed Accessions from Sichuan Province, by Kompetitive Allele-Specific PCR

文献类型： 外文期刊

作者： Chai, Liang 1 ; Zhang, Jinfang 1 ; Fernando, Wannakuwattewaduge Gerard Dilantha 2 ; Li, Haojie 1 ; Huang, Xiaoqi 1 ;

作者机构： 1.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China

2.Univ Manitoba, Dept Plant Sci, Winnipeg, MB R3T 2N2, Canada

3.Sichuan Acad Agr Sci, Inst Plant Protect, Chengdu 610066, Sichuan, Peoples R China

关键词： blackleg resistance; double-low rapeseed; KASP; Leptosphaeria maculans; Rlm1 gene

期刊名称：PLANT PATHOLOGY JOURNAL （ 影响因子：1.57； 五年影响因子：1.775 ）

ISSN： 1598-2254

年卷期： 2021 年 37 卷 2 期

页码：

收录情况： SCI

摘要： Blackleg is a serious disease in Brassica plants, causing moderate to severe yield losses in rapeseed worldwide. Although China has not suffered from this disease yet (more aggressive Leptosphaeria maculans is not present yet), it is crucial to take provisions in breeding for disease resistance to have excellent blackleg-resistant cultivars already in the fields or in the breeding pipeline. The most efficient strategy for controlling this disease is breeding plants with identified resistance genes. We selected 135 rapeseed accessions in Sichuan, including 30 parental materials and 105 hybrids, and we determined their glucosinolate and erucic acid content and confirmed 17 double-low materials. A recently developed single-nucleotide polymorphism (SNP) marker, SNP_208, was used to genotype allelic Rlm1/rlm1 on chromosome A07, and 87 AvrLm1-resistant materials. Combined with the above-mentioned seed quality data, we identified 11 AvrLm1-resistant double-low rapeseed accessions, including nine parental materials and two hybrids. This study lays the foundation of specific R gene-oriented breeding, in the case that the aggressive Leptosphaeria maculans invades and establishes in China in the future and a robust and less labor consuming method to identify resistance in canola germplasm.

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