Full-length transcriptome sequencing and identification of immune-related genes in the critically endangered Hucho bleekeri
文献类型: 外文期刊
作者: Chen, Yeyu 1 ; Yang, Huanchao 1 ; Chen, Yanling 1 ; Song, Mingjiang 1 ; Liu, Bo 2 ; Song, Jingguo 3 ; Liu, Xin 3 ; Li, Hu 1 ;
作者机构: 1.Sichuan Acad Agr Sci, Fishery Inst, Chengdu 611730, Peoples R China
2.Yaan Fishery Dev Ctr, Yaan 625000, Peoples R China
3.Sichuan Zumuzu River Basin Hydropower Dev Co Ltd, Chengdu 610094, Peoples R China
关键词: Hucho bleekeri; Isoform sequencing; Illumina sequencing; Immune-related genes
期刊名称:DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY ( 影响因子:3.636; 五年影响因子:3.654 )
ISSN: 0145-305X
年卷期: 2021 年 116 卷
页码:
收录情况: SCI
摘要: Hucho bleekeri is a glacial relict and critically endangered fish restricted to the Yangtze River drainage in China. The lack of basic genomic information and immune characteristics will hinder the way toward protecting this species. In the present study, we conducted the first transcriptome analysis of H. bleekeri using the combination of SMRT and Illumina sequencing technology. Transcriptome sequencing generated a total of 93,330 nonredundant full-length unigenes with a mean length of 3072 bp. A total of 92,472 (99.08%) unigenes were annotated in at least one of the Nr protein, Swiss-Prot, KEGG, KOG, GO, Nt and Pfam databases. KEGG analysis showed that a total of 7240 unigenes belonging to 28 immune pathways were annotated to the immune system category. Meanwhile, differentially expressed genes between mucosa-associated tissues (skin, gill and hindgut) and systemic-immune tissues (spleen, head kidney and liver) were obtained. Importantly, genes participating in diverse immune signalling pathways and their expression profiles in H. bleekeri were discussed. In addition, a large number of long non-coding RNAs (lncRNAs) and simple sequence repeats (SSRs) were obtained in the H. bleekeri transcriptome. The present study will provide basic genomic information for H. bleekeri and for further research on analysing the characteristics of both the innate and adaptive immune systems of this critically endangered species.
- 相关文献
作者其他论文 更多>>
-
Comparison of muscle structure and transcriptome analysis reveals the mechanism of growth variation in Yangtze sturgeon (Acipenser dabryanus)
作者:Li, Qingzhi;Chen, Yeyu;Wu, Xiaoyun;Liu, Ya;Lai, Jiansheng;Song, Mingjiang;Li, Feiyang;Li, Pengcheng;He, Bin;Wang, Bin;Yan, Tao;Gong, Quan;Li, Qingzhi;Wang, Bin;Gong, Quan;Wang, Jun;Wang, Jun
关键词:Acipenser dabryanus; Growth difference; GH/IGF axis; Myogenic regulatory factor; Cell cycle
-
Biochemical, transcriptomic and metabolomic responses to total dissolved gas supersaturation and their underlying molecular mechanisms in Yangtze sturgeon (Acipenser dabryanus)
作者:Chen, Yeyu;Wu, Xiaoyun;Lai, Jiansheng;Liu, Ya;Song, Mingjiang;Li, Feiyang;Gong, Quan;Liu, Xiaoqing
关键词:Acipenser dabryanus; Total dissolved gas; Oxidative response; Heat shock protein; Energy metabolism; Immunity
-
Effect of Crop Rotation on Fusarium Mycotoxins and Fusarium Species in Cereals in Sichuan Province (China)
作者:Dong, Fei;Wu, Deliang;Shen, Guanghui;Liu, Xin;Xu, Jian Hong;Shi, Jian Rong;Dong, Fei;Mokoena, Mduduzi P.;Olaniran, Ademola O.;Shi, Jian Rong;Chen, Xiangxiang;Liu, Xin;Xu, Jian Hong;Shi, Jian Rong;Lei, Xinyu;Zhang, Yifan;Li, Ying;Lee, Yin-Won
关键词:crop rotation; Fusarium; Fusarium species; pathogenicity
-
Identification of the Solid Stem Suppressor Gene Su-TdDof in Synthetic Hexaploid Wheat Syn-SAU-117
作者:Li, Hui;Huang, Lin;Liu, Dengcai;Zhang, Lianquan;Li, Hui;Liu, Xin;Zhang, Junqing;Chen, Longyu;Zhang, Minghu;Miao, Yongping;Ma, Pan;Hao, Ming;Jiang, Bo;Ning, Shunzong;Huang, Lin;Yuan, Zhongwei;Chen, Xuejiao;Chen, Xue;Liu, Dengcai;Zhang, Lianquan;Wan, Hongshen;Wan, Hongshen
关键词:semisolid; gene mapping; Aegilops tauschii; synthetic hexaploid wheat; lodging resistance
-
Comparative transcriptome analysis provides insights into the TDG supersaturation stress response of Schizothorax davidi
作者:Chen, Yeyu;Wu, Xiaoyun;Lai, Jiansheng;Gong, Quan;Liu, Xiaoqing
关键词:Environmental stress; Cell cycle; Apoptosis; Immunity
-
Transcriptome sequencing of the spleen of the Yangtze sturgeon (Acipenser dabryanus) under Edwardsiella tarda and poly(I:C) treatments
作者:Gong, Quan;Chen, Yeyu;Wu, Xiaoyun;Lai, Jiansheng;Liu, Ya;Song, Mingjiang;Li, Feiyang
关键词:Acipenser dabryanus; SMRT sequencing; Illumina sequencing; Immunity -related genes; Metabolism
-
Isolation and characterization of 73 SNP markers in Schizopygopsis chengi baoxingensis
作者:Song, Mingjiang;Chen, Yeyu;Wu, Xiaoyun;Liu, Ya;Li, Feiyang;Gong, Quan;Ou, Jun;Li, Pengcheng;Zou, Qiaolin;Lai, Jiansheng
关键词:Schizopygopsis chengi baoxingensis; SNP marker; Genetic diversity analysis; RAD-Seq