Transcriptomic analysis of Mythimna separata ovaries and identification of genes involved in reproduction
文献类型： 外文期刊
作者： Fu, Junhong 1 ; Li, Lingling 2 ; Dai, Changgeng 2 ; Zhang, Ying 2 ; Hu, Yang 2 ; Hu, Chaoxing 1 ; Li, Hongbo 2 ;
作者机构： 1.Guizhou Univ, Inst Entomol, Sch Agr, Guizhou Prov Key Lab Agr Pest Management Mountaino, Guiyang 550025, Peoples R China
2.Guizhou Acad Agr Sci, Inst Plant Protect, Guiyang 550006, Guizhou, Peoples R China
关键词： Mythimna separata; Transcriptome; Ovary development; Gene expression
期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 影响因子：3.0； 五年影响因子：3.2 ）
ISSN： 1744-117X
年卷期： 2023 年 46 卷
页码：
收录情况： SCI
摘要： The migratory insect Mythimna separata is a major pest of grain crops in Asia. Unfortunately, the molecular mechanisms that control and regulate reproduction in this species remain unclear. In this study, transcriptome sequencing was utilized to identify genes associated with ovary development and oogenesis. Clean sequences totaling 117.71 Gb were assembled into 178,534 unigenes with a mean length of 647.37 bp and N50 length of 837 bp. Transcriptome analysis showed that 7921 unigenes were significantly expressed in ovaries with 4403 and 3518 unigenes up- and down-regulated, respectively. Enrichment analysis with the Kyoto Encyclopedia of Genes and Genomes database suggested that 729 differentially expressed genes were significantly enriched in the top 20 pathways (q-values <0.05). Twenty genes were associated with ovary development and oogenesis and included lipases, Nanos, small heat shock proteins (sHsps) and histones; these were further verified by qRT-PCR and may play essential roles in M. separata reproduction. Collectively, our findings reveal underlying mechanisms of M.separata reproduction and may lead to RNAi-based management strategies targeting reproductive physiology.
- 相关文献
作者其他论文 更多>>
-
The long-acting herbicide mesosulfuron-methyl inhibits soil microbial community assembly mediating nitrogen cycling
作者：Cao, Junli;Zhang, Ying;Dai, Gaochen;Cui, Kai;Wu, Xiaohu;Xu, Jun;Dong, Fengshou;Pan, Xinglu;Zheng, Yongquan;Cao, Junli;Zhang, Ying;Dai, Gaochen;Qin, Fanxin
关键词：Mesosulfuron-methyl; Soil N -cycle functional genes; Microbial community assembly; Network modules; Structural equation model
-
Dissipation and dietary risk assessment of cyflumetofen, bifenazate and their metabolites in citrus in China
作者：Zhang, Ying;Wang, Weitao;Huang, Wenyuan;Mao, Jiayi;Wang, Huaixu;Zhang, Ying;Zhang, Xinzhong;Kaium, Abdul
关键词：cyflumetofen; bifenazate; metabolite; citrus; dietary risk assessment
-
Exogenous application of nutrient elements effectively reduces grape cracking and improves fruit quality
作者：Zhang, Weilong;Zhang, Qi;Liang, Fahui;Yang, Jinghui;Song, Pingli;Li, Gang;Wang, Enquan;Lv, Zhengxin;Zhang, Ying
关键词："Muscat Hamburg"; "Jumeigui"; Microelements; Grape cracking; Fruit quality
-
Occurrence and impact of carbendazim and hymexazol residues on yeast growth and ochratoxin A contamination during wine production
作者：Zheng, Yongquan;Zhang, Ying;Zheng, Yongquan;Zhang, Ying;Duan, Tingting;Wei, Dongmei;Wu, Xiaohu;Xu, Jun;Dong, Fengshou;Pan, Xinglu;Wei, Dongmei;Wu, Xiaohu;Xu, Jun;Dong, Fengshou;Pan, Xinglu
关键词：Aspergillus carbonarius; carbendazim; hymexazol; ochratoxin A; vinification
-
Genetic diversity of RNA viruses infecting invertebrate pests of rice
作者：Wang, Haoran;Chao, Shufen;Yan, Qing;Chen, Guoqing;Mao, Chonghui;Yang, Baojun;He, Jiachun;Feng, Guozhong;Wang, Haoran;Zhang, Shu;Lv, Liang;Hu, Yang;Yu, Fengquan;Wang, Shuo;Zhang, Songbai;Zhang, Liangsheng;Zhang, Liangsheng;Simmonds, Peter
关键词：invertebrate species; metatranscriptome; viral diversity; viral transmission; viral genomes
-
Application of thifluzamide alters microbial network structure and affects methane cycle genes in rice-paddy soil
作者：Zhang, Ying;Li, Xiaogang;Zhang, Ying;Wu, Xiaohu;Xu, Jun;Dong, Fengshou;Liu, Xingang;Zheng, Yongquan;Zhang, Ying;Chen, Caijun
关键词：Thifluzamide; Community structure; Network; Methane
-
Seed Treatment with Diamide and Neonicotinoid Mixtures for Controlling Fall Armyworm on Corn: Toxicity Evaluation, Effects on Plant Growth and Residuality
作者：Li, Hongbo;Zhang, Ying;Huang, Wenyuan;Duan, Tingting;Hu, Yang;Feng, Lei;Fu, Junhong;Xing, Jichun
关键词：Spodoptera frugiperda; diamide; neonicotinoid; seed treatment; combined toxicity
