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Transcriptome profiling of genes associated with fruit firmness in the melon variety 'Baogua' (Cucumis melo ssp. agrestis Jeffrey)

文献类型： 外文期刊

作者： Zhang, Huijun 1 ; Zhang, Yan 1 ; Wang, Pengcheng 3 ; Zhang, Jian 3 ;

作者机构： 1.Huaibei Normal Univ, Sch Life Sci, Huaibei 235000, Anhui, Peoples R China

2.Anhui Jianghuai Hort Seeds Co Ltd, Anhui Prov Key Lab Pollutant Sensit Mat & Environ, Huaibei 235000, Anhui, Peoples R China

3.Anhui Acad Agr Sci, Inst Hort, Hefei 230031, Anhui, Peoples R China

4.Key Lab Intelligent Seedling Breeding Vegetable F, Maanshan 238200, Anhui, Peoples R China

5.Key Lab Genet Improvement & Ecophysiol Hort Crops, Hefei 230031, Anhui, Peoples R China

关键词： beta-galactosidase; Baogua melon; Fruit firmness; Subcellular location; Transcriptome sequencing

期刊名称：PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS （ 影响因子：3.023； 五年影响因子：3.442 ）

ISSN： 0971-5894

年卷期： 2022 年 28 卷 2 期

页码：

收录情况： SCI

摘要： Fruit firmness is an important trait of melons due to its effect on fresh fruit consumption, storage, and transport. However, information on the expression of genes influencing the fruit firmness of 'Baogua' (BG) melon (Cucutnis melo ssp. agrestis Jeffrey) remains rare. This study aimed to identify the key genes associated with the firmness of BG fruit sampled at 14 and 28 days after pollination (dap) via transcriptome sequencing. A total of 1113 up-regulated and 2224 down-regulated differentially expressed genes (DEGs) were identified. The main Gene Ontology terms assigned to the DEGs were phosphotransferase activity, alcohol group as acceptor, protein phosphorylation, and protein kinase activity. The enriched KEGG pathways involving the DEGs were starch and sucrose metabolism, diterpenoid biosynthesis, plant hormone signal transduction, and MAPK signaling pathway- plant. In addition, qRT-PCR verified that four GAL genes, namely, CmGAL1-4, were differentially expressed at 0, 7, 14, 21, and 28 dap. Our data revealed that CmGAL1 expression was highest at 21 dap. However, the expression levels of CmGAL2-4 were highest at 14 dap. The sequence of CinGAL1 was similar to the sequences of homologs from melon and cucumber. Subcellular localization analysis revealed CmGAL1 was located in the cell membrane and cytoplasm. Our findings implied that fruit development at 14 dap, which is a key time-point, varies considerably from fruit development at 28 dap. Our present study provides new information on the genes associated with BG fruit firmness and help improve the storage and transport of BG fruit prior to processing.

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