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Analysis of cashmere goat meat by label-free proteomics shows that MYL3 is a potential molecular marker of meat toughness

文献类型: 外文期刊

作者: Xie, Yuchun 1 ; Rile, Nai 4 ; Li, Xuewu 5 ; Li, Haijun 6 ; Zhao, Meng 7 ; Che, Tianyu 1 ; Cai, Ting 8 ; Liu, Zhihong 1 ; Li, Jinquan 1 ;

作者机构: 1.Inner Mongolia Agr Univ, Coll Anim Sci, Hohhot, Peoples R China

2.Key Lab Anim Genet Breeding & Reprod, Hohhot, Inner Mongolia, Peoples R China

3.Minist Agr, Key Lab Mutton Sheep Genet & Breeding, Hohhot, Peoples R China

4.Hulunbuir Univ, Sch Agr & Forestry, Hulunbuir, Peoples R China

5.Panzhihua Univ, Sch Hlth & Wellness, Panzhihua, Peoples R China

6.Inner Mongolia Agr Univ, Coll Vet Med, Hohhot, Peoples R China

7.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot, Peoples R China

8.Inner Mongolia Autonomous Reg Agr & Anim Husb Tec, Hohhot, Peoples R China

关键词: SWATH; interaction network; muscle fibre type

期刊名称:CZECH JOURNAL OF ANIMAL SCIENCE ( 影响因子:1.349; 五年影响因子:1.387 )

ISSN: 1212-1819

年卷期: 2022 年 67 卷 4 期

页码:

收录情况: SCI

摘要: The Inner Mongolia cashmere goat is famous for its bright white cashmere fibre. However, little attention is given to the excellent characteristics of this breed's meat. We used label-free proteomics to analyse the total protein content in five different muscles, and 1 227 proteins were detected. Through sequential windowed acquisition of all theoretical fragment ions (SWATH), 16, 33, 49, 39, and 31 differentially expressed proteins were successfully detected in the five muscles. Protein-protein interaction network analysis of differentially expressed proteins revealed many strong interactions related to fatty acid beta oxidation and muscle development. Based on SWATH in five muscles, 25 differentially expressed proteins related to muscle development were detected, including seven muscle fibre analysis of MYL3 showed that the proportion of MYL3 may be a potential molecular marker for muscle toughness.

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