Efficient 2,3-Butanediol/Acetoin Production Using Whole-Cell Biocatalyst with a New Nadh/Nad(+) Regeneration System
文献类型: 外文期刊
作者: Wang, Yaping 1 ; Peng, Yanhong 1 ; Liu, Xiaoyan 1 ; Zhou, Ronghua 1 ; Liao, Xianqing 1 ; Min, Yong 1 ; Hu, Yong 3 ; Wang, Ying 1 ; Rao, Ben 1 ;
作者机构: 1.Hubei Acad Agr Sci, Natl Biopesticide Engn Technol Res Ctr, Hubei Innovat Ctr Agr Sci & Technol, Hubei Biopesticide Engn Res Ctr,Biopesticide Bran, Wuhan 430064, Peoples R China
2.Hubei Univ, Engn Hubei Collaborat Innovat Ctr Green Transform, State Key Lab Biocatalysis & Enzyme, Hubei Key Lab Ind Biotechnol,Biol Fac, Wuhan 430062, Peoples R China
3.Hubei Univ Technol, Natl 111 Ctr Cellular Regulat & Mol Pharmaceut, Hubei Res Ctr Food Fermentat Engn & Technol, Minist Educ,Key Lab Fermentat Engn,Hubei Key Lab, Wuhan 430068, Peoples R China
关键词: 2; 3-butanediol; acetoin; Serratia marcescens; whole-cell biocatalysts; NADH; NAD(+) regeneration system
期刊名称:CATALYSTS ( 影响因子:4.501; 五年影响因子:4.641 )
ISSN:
年卷期: 2021 年 11 卷 12 期
页码:
收录情况: SCI
摘要: An auto-inducing expression system was developed that could express target genes in S. marcescens MG1. Using this system, MG1 was constructed as a whole-cell biocatalyst to produce 2,3-butanediol/acetoin. Formate dehydrogenase (FDH) and 2,3-butanediol dehydrogenase were expressed together to build an NADH regeneration system to transform diacetyl to 2,3-butanediol. After fermentation, the extract of recombinant S. marcescens MG1ABC (pETDuet-bdhA-fdh) showed 2,3-BDH activity of 57.8 U/mg and FDH activity of 0.5 U/mg. And 27.95 g/L of 2,3-BD was achieved with a productivity of 4.66 g/Lh using engineered S. marcescens MG1(Pswnb+pETDuet-bdhA-fdh) after 6 h incubation. Next, to produce 2,3-butanediol from acetoin, NADH oxidase and 2,3-butanediol dehydrogenase from Bacillus subtilis were co-expressed to obtain a NAD+ regeneration system. After fermentation, the recombinant strain S. marcescens MG1ABC (pSWNB+pETDuet-bdhA-yodC) showed AR activity of 212.4 U/mg and NOX activity of 150.1 U/mg. We obtained 44.9 g/L of acetoin with a productivity of 3.74 g/Lh using S. marcescens MG1ABC (pSWNB+pETDuet-bdhA-yodC). This work confirmed that S. marcescens could be designed as a whole-cell biocatalyst for 2,3-butanediol and acetoin production.
- 相关文献
作者其他论文 更多>>
-
Preparation of granite powder-based geopolymer by synergistic action of calcination and phosphoric acid
作者:Jia, Luxia;Huang, Min;Huang, Muyang;Luo, Yongpeng;Zhang, Xiuze;Bao, Shenxu;Huang, Min;Bao, Shenxu;Liao, Xianqing;Bao, Shenxu
关键词:calcination; geopolymer; granite powder; phosphoric acid; pore structure
-
Novel Steroidal[17,16-d]pyrimidines Derived from Epiandrosterone and Androsterone: Synthesis, Characterization and Configuration-Activity Relationships
作者:Yang, Fei;Yang, Ziwen;Yang, Fei;Liu, Fang;Min, Yong;Shi, Liqiao;Liu, Manli;Wang, Kaimei;Ke, Shaoyong;Gong, Yan;Yang, Ziwen
关键词:epiandrosterone; androsterone; steroidal[17; 16-d]pyrimidines; synthesis; bioactivity; molecular docking; SARs
-
Improving the Activity of Tryptophan Synthetase via a Nucleic Acid Scaffold
作者:Wang, Yaping;Wang, Xiangyi;Niu, Shuhui;Cheng, Wei;Liu, Xiaoyan;Min, Yong;Qiu, Yimin;Rao, Ben;Zhu, Lei;Wang, Yaping;Wang, Xiangyi;Niu, Shuhui;Cheng, Wei;Ma, Lixin
关键词:tryptophan synthetase; dCE; TrapA; TrapB; nucleic acid scaffold
-
Acaricidal Activity and Field Efficacy Analysis of the Potential Biocontrol Agent Bacillus vallismortis NBIF-001 against Spider Mites
作者:Zhu, Lei;Chen, Ling;Min, Yong;Liu, Fang;Liao, Xianqing;Rao, Ben;Qiu, Yimin;Chen, Wei;Wang, Kaimei;Yang, Ziwen;Zhou, Ronghua;Gong, Yan;Liu, Xiaoyan;Zhu, Lei;Chen, Ling;Min, Yong;Liu, Fang;Liao, Xianqing;Rao, Ben;Qiu, Yimin;Chen, Wei;Wang, Kaimei;Yang, Ziwen;Zhou, Ronghua;Gong, Yan;Liu, Xiaoyan;Zhu, Lei;Chen, Ling;Min, Yong;Liu, Fang;Liao, Xianqing;Rao, Ben;Qiu, Yimin;Chen, Wei;Wang, Kaimei;Yang, Ziwen;Zhou, Ronghua;Gong, Yan;Liu, Xiaoyan;Zhou, Ronghua
关键词:spider mites; Bacillus vallismortis; acaricidal activity; field efficacy; protein BVP8; biocontrol
-
Complete Genome Sequence of Brachybacterium sp. Strain NBEC-018, Isolated from Nematode-Infected Potatoes
作者:Chen, Ling;Wang, Yueying;Liu, Nanxi;Zhu, Lei;Min, Yong;Qiu, Yimin;Tian, Yuxi;Liu, Xiaoyan
关键词:
-
Expression and Surface Display of an Acidic Cold-Active Chitosanase in Pichia pastoris Using Multi-Copy Expression and High-Density Cultivation
作者:Peng, Yanhong;Wang, Yaping;Liu, Xiaoyan;Zhou, Ronghua;Liao, Xianqing;Min, Yong;Wang, Ying;Rao, Ben;Peng, Yanhong;Wang, Yaping;Ma, Lixin
关键词:chitosanase; chiton oligosaccharides; high-density fermentation; multi-copy strains; Pichia pastoris; displayed enzyme
-
Carboline derivatives based on natural pityriacitrin as potential antifungal agents
作者:Huang, Daye;Zhang, Zhigang;Liu, Fang;Huang, Wenbo;Min, Yong;Wang, Kaimei;Yang, Jingzhong;Cao, Chunxia;Gong, Yan;Ke, Shaoyong;Huang, Daye;Zhang, Zhigang;Liu, Fang;Huang, Wenbo;Min, Yong;Wang, Kaimei;Yang, Jingzhong;Cao, Chunxia;Gong, Yan;Ke, Shaoyong;Li, Yanyan
关键词:Carboline alkaloids; Carboline derivatives; Antifungal activity; Broad-spectrum