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High-Quality Genome Assembly and Genome-Wide Association Study of Male Sterility Provide Resources for Flax Improvement

文献类型： 外文期刊

作者： Zhao, Xiaoqing 1 ; Yi, Liuxi 2 ; Zuo, Yongchun 3 ; Gao, Fengyun 1 ; Cheng, Yuchen 1 ; Zhang, Hui 1 ; Zhou, Yu 1 ; Jia, Xiaoyun 1 ; Su, Shaofeng 1 ; Zhang, Dejian 3 ; Zhang, Xiangqian 1 ; Ren, Yongfeng 1 ; Mu, Yanxin 1 ; Jin, Xiaolei 1 ; Li, Qiang 1 ; Bateer, Siqin 1 ; Lu, Zhanyuan 1 ;

作者机构： 1.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010031, Peoples R China

2.Inner Mongolia Agr Univ, Agr Coll, Hohhot 010019, Peoples R China

3.Inner Mongolia Univ, Coll Life Sci, Hohhot 010070, Peoples R China

4.State Key Lab Reprod Regulat & Breeding Grassland, Hohhot 010019, Peoples R China

5.Inner Mongolia Key Lab Degradat Farmland Ecol Rest, Hohhot 010031, Peoples R China

6.Inner Mongolia Conservat Tillage Engn Technol Res, Hohhot 010031, Peoples R China

关键词： flax; genome assembly; male sterility; GWAS

期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）

ISSN：

年卷期： 2023 年 12 卷 15 期

页码：

收录情况： SCI

摘要： Flax is an economic crop with a long history. It is grown worldwide and is mainly used for edible oil, industry, and textiles. Here, we reported a high-quality genome assembly for "Neiya No. 9", a popular variety widely grown in China. Combining PacBio long reads, Hi-C sequencing, and a genetic map reported previously, a genome assembly of 473.55 Mb was constructed, which covers similar to 94.7% of the flax genome. These sequences were anchored onto 15 chromosomes. The N50 lengths of the contig and scaffold were 0.91 Mb and 31.72 Mb, respectively. A total of 32,786 protein-coding genes were annotated, and 95.9% of complete BUSCOs were found. Through morphological and cytological observation, the male sterility of flax was considered dominant nuclear sterility. Through GWAS analysis, the gene LUSG00017705 (cysteine synthase gene) was found to be closest to the most significant SNP, and the expression level of this gene was significantly lower in male sterile plants than in fertile plants. Among the significant SNPs identified in the GWAS analysis, only two were located in the coding region, and these two SNPs caused changes in the protein encoded by LUSG00017565 (cysteine protease gene). It was speculated that these two genes may be related to male sterility in flax. This is the first time the molecular mechanism of male sterility in flax has been reported. The high-quality genome assembly and the male sterility genes revealed, provided a solid foundation for flax breeding.

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