Establishment and functional characterization of immortalized rabbit dermal papilla cell lines
文献类型: 外文期刊
作者: Li, Jiali 1 ; Zhao, Bohao 1 ; Zhang, Xiyu 1 ; Dai, Yingying 1 ; Yang, Naisu 1 ; Bao, Zhiyuan 1 ; Chen, Yang 1 ; Liu, Yan 2 ; Wu, Xinsheng 1 ;
作者机构: 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou, Jiangsu, Peoples R China
2.Zhejiang Acad Agr Sci, Anim Husb & Vet Res Inst, Hangzhou, Zhejiang, Peoples R China
3.Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou, Jiangsu, Peoples R China
关键词: Dermal papilla cell; SV40T; Immortalization; Rabbit
期刊名称:ANIMAL BIOTECHNOLOGY ( 影响因子:3.7; 五年影响因子:3.3 )
ISSN: 1049-5398
年卷期: 2023 年
页码:
收录情况: SCI
摘要: Hair follicle (HF) undergo periodic growth and development in mammals, which regulated by dermal papilla cells (DPCs) are reported to play an important role in HF morphogenesis and development. However, primary DPCs have low proliferative activity, age quickly, and fresh cell isolation is both time-consuming and laborious. In this study, we introduced the SV40 large T antigen (SV40T) into dissociated early passage rabbit vibrissae DPCs with lentiviral vectors and established seven immortalized DPC lines (R-1, R-2, R-3, R-4, R-5, R-6 and R-7). These cell lines displayed early passage morphology and high alkaline phosphatase activity. RT-PCR and immunofluorescence staining showed that all the immortalized cell lines expressed the DPC markers (& alpha;-SMA, IGF1, ALPL, FGF2, BMP2 and TGF & beta;2), but & alpha;-SMA was only expressed well in R-3, R-4, and R-7. Furthermore, it was found that R-7 was the only line to survive beyond 50 passages. Compared to melanoma cells, R-7 did not undergo malignant transformation. Karyotyping and cell growth viability analysis illustrated that the R-7 cell line preserved the basic characteristics of primary DPCs. The R-7 DPCs established have potential application for future hair research. The study provides the theoretical basis in the cell research of HF growth and development.
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