Mechanism of Inhibiting the Growth and Aflatoxin B-1 Biosynthesis of Aspergillus flavus by Phenyllactic Acid
文献类型： 外文期刊
作者： Zhao, Chi 1 ; Penttinen, Petri 1 ; Zhang, Lingzi 1 ; Dong, Ling 2 ; Zhang, Fengju 2 ; Li, Zhihua 2 ; Zhang, Xiaoping 1 ;
作者机构： 1.Sichuan Agr Univ, Coll Resources, 211 Huimin Rd, Chengdu 611130, Peoples R China
2.Sichuan Acad Agr Sci, Inst Agroprod Proc Sci & Technol, 60 Shizishan Rd, Chengdu 610066, Peoples R China
3.Univ Helsinki, Fac Agr & Forestry, Viikinkaari 1, Helsinki 00014, Finland
关键词： phenyllactic acid; Aspergillus flavus; aflatoxin B-1; transcriptome; metabolome
期刊名称：TOXINS （ 影响因子：4.2； 五年影响因子：4.7 ）
ISSN：
年卷期： 2023 年 15 卷 6 期
页码：
收录情况： SCI
摘要： Phenyllactic acid (PLA), a promising food preservative, is safe and effective against a broad spectrum of food-borne pathogens. However, its mechanisms against toxigenic fungi are still poorly understood. In this study, we applied physicochemical, morphological, metabolomics, and transcriptomics analyses to investigate the activity and mechanism of PLA inhibition of a typical food-contaminating mold, Aspergillus flavus. The results showed that PLA effectively inhibited the growth of A. flavus spores and reduced aflatoxin B-1 (AFB(1)) production by downregulating key genes associated with AFB(1) biosynthesis. Propidium iodide staining and transmission electron microscopy analysis demonstrated a dose-dependent disruption of the integrity and morphology of the A. flavus spore cell membrane by PLA. Multi-omics analyses showed that subinhibitory concentrations of PLA induced significant changes in A. flavus spores at the transcriptional and metabolic levels, as 980 genes and 30 metabolites were differentially expressed. Moreover, KEGG pathway enrichment analysis indicated PLA-induced cell membrane damage, energy-metabolism disruption, and central-dogma abnormality in A. flavus spores. The results provided new insights into the anti-A. flavus and -AFB(1) mechanisms of PLA.
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