Optimization of Extraction Process, Structure Characterization, and Antioxidant Activity of Polysaccharides from Different Parts of Camellia oleifera Abel
文献类型: 外文期刊
作者: Feng, Shiling 1 ; Tang, Min 1 ; Jiang, Zhengfeng 1 ; Ruan, Yunjie 1 ; Liu, Li 1 ; Kong, Qingbo 1 ; Xiang, Zhuoya 1 ; Chen, Tao 1 ; Zhou, Lijun 1 ; Yang, Hongyu 1 ; Yuan, Ming 1 ; Ding, Chunbang 1 ;
作者机构: 1.Sichuan Agr Univ, Coll Life Sci, Yaan 625014, Peoples R China
2.Sichuan Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Chengdu 610066, Peoples R China
关键词: Camellia oleifera; Box-Behnken design; physicochemical property; antioxidant activity
期刊名称:FOODS ( 影响因子:5.561; 五年影响因子:5.94 )
ISSN:
年卷期: 2022 年 11 卷 20 期
页码:
收录情况: SCI
摘要: The flowers, leaves, seed cakes and fruit shells of Camellia oleifera are rich in bioactive polysaccharides, which can be used as additives in food and other industries. In this study, a Box-Behnken design was used to optimize the extraction conditions of polysaccharides from C. oleifera flowers (P-CF), leaves (P-CL), seed cakes (P-CC), and fruit shells (P-CS). Under the optimized extraction conditions, the polysaccharide yields of the four polysaccharides were 9.32% +/- 0.11 (P-CF), 7.57% +/- 0.11 (P-CL), 8.69% +/- 0.16 (P-CC), and 7.25% +/- 0.07 (P-CS), respectively. Polysaccharides were mainly composed of mannose, rhamnose, galacturonic acid, glucose, galactose, and xylose, of which the molecular weights ranged from 3.31 kDa to 128.06 kDa. P-CC had a triple helix structure. The antioxidant activities of the four polysaccharides were determined by Fe2+ chelating and free radical scavenging abilities. The results showed that all polysaccharides had antioxidant effects. Among them, P-CF had the strongest antioxidant activity, of which the highest scavenging ability of DPPH center dot, ABTS(center dot+), and hydroxyl radical could reach 84.19% +/- 2.65, 94.8% +/- 0.22, and 79.97% +/- 3.04, respectively, and the best chelating ability of Fe2+ could reach 44.67% +/- 1.04. Overall, polysaccharides extracted from different parts of C. oleifera showed a certain antioxidant effect, and could be developed as a new type of pure natural antioxidant for food.
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